Bromodomains regulate dynamic targeting of the PBAF chromatin remodeling complex to chromatin hubs.

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Biophysical Journal. 2022 May 3;121(9):1738-1752. doi: 10.1016/j.bpj.2022.03.027

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Bromodomains regulate dynamic targeting of the PBAF chromatin remodeling complex to chromatin hubs. Lavis Lab

Kenworthy CA, Haque N, Liou S, Chandris P, Wong V, Dziuba P, Lavis LD, Liu W, Singer RH, Coleman RA

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Abstract

Chromatin remodelers actively target arrays of acetylated nucleosomes at select enhancers and promoters to facilitate or shut down the repeated recruitment of RNA Pol II during transcriptional bursting. It is poorly understood how chromatin remodelers such as PBAF dynamically target different chromatin states inside a live cell. Our live-cell single molecule fluorescence microscopy study reveals chromatin hubs throughout the nucleus where PBAF rapidly cycles on and off the genome. Deletion of PBAF's bromodomains impairs targeting and stable engagement of chromatin in hubs. Dual color imaging reveals that PBAF targets both euchromatic and heterochromatic hubs with distinct genome binding kinetic profiles that mimic chromatin stability. Removal of PBAF's bromodomains stabilizes H3.3 binding within chromatin indicating that bromodomains may play a direct role in remodeling of the nucleosome. Our data suggests that PBAF's dynamic bromodomain mediated engagement of a nucleosome may reflect the chromatin remodeling potential of differentially bound chromatin states.

PMID: 35364106 [PubMed - indexed for MEDLINE]

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Previous bioRxiv PrePrint https://doi.org/10.1101/111674

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Bromodomains regulate dynamic targeting of the PBAF chromatin remodeling complex to chromatin hubs.

Abstract

PMID: 35364106 [PubMed - indexed for MEDLINE]

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