Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy.

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Neuron. 2020 May 06;106(3):369-387. doi: 10.1016/j.neuron.2020.03.004

Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy. Keller Lab

Ueda HR, Dodt H, Osten P, Economo MN, Chandrashekar J, Keller PJ

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Abstract

Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields have been combined only recently to facilitate novel experiments and measurements in neuroscience. Since tissue-clearing methods were first combined with modernized light-sheet microscopy a decade ago, the performance of both technologies has rapidly improved, broadening their applications. Here, we review the state of the art of tissue-clearing methods and light-sheet microscopy and discuss applications of these techniques in profiling cells and circuits in mice. We examine outstanding challenges and future opportunities for expanding these techniques to achieve brain-wide profiling of cells and circuits in primates and humans. Such integration will help provide a systems-level understanding of the physiology and pathology of our central nervous system.

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Whole-brain profiling of cells and circuits in mammals by tissue clearing and light-sheet microscopy.

Abstract

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