An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae

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bioRxiv. 2024 Aug 19;. doi: 10.1101/2024.08.16.608298

An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae Scientific Computing Software

Yamaguchi N, Otsuna H, Eisenberg-Bord M, Ramakrishnan L

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Abstract

Zebrafish larvae are used to model the pathogenesis of multiple bacteria. This transparent model offers the unique advantage of allowing quantification of fluorescent bacterial burdens (fluorescent pixel counts: FPC) in vivo by facile microscopical methods, replacing enumeration of bacteria using time-intensive plating of lysates on bacteriological media. Accurate FPC measurements require laborious manual image processing to mark the outside borders of the animals so as to delineate the bacteria inside the animals from those in the culture medium that they are in. Here, we have developed an automated ImageJ/Fiji-based macro that accurately detect the outside borders of Mycobacterium marinum-infected larvae.

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An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae

Abstract

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