Filter
Associated Lab
- Aguilera Castrejon Lab (1) Apply Aguilera Castrejon Lab filter
- Ahrens Lab (4) Apply Ahrens Lab filter
- Aso Lab (6) Apply Aso Lab filter
- Betzig Lab (4) Apply Betzig Lab filter
- Branson Lab (2) Apply Branson Lab filter
- Card Lab (6) Apply Card Lab filter
- Clapham Lab (1) Apply Clapham Lab filter
- Darshan Lab (4) Apply Darshan Lab filter
- Dickson Lab (4) Apply Dickson Lab filter
- Dudman Lab (2) Apply Dudman Lab filter
- Espinosa Medina Lab (1) Apply Espinosa Medina Lab filter
- Feliciano Lab (1) Apply Feliciano Lab filter
- Fitzgerald Lab (5) Apply Fitzgerald Lab filter
- Funke Lab (12) Apply Funke Lab filter
- Harris Lab (7) Apply Harris Lab filter
- Hermundstad Lab (2) Apply Hermundstad Lab filter
- Hess Lab (6) Apply Hess Lab filter
- Jayaraman Lab (1) Apply Jayaraman Lab filter
- Karpova Lab (2) Apply Karpova Lab filter
- Keller Lab (1) Apply Keller Lab filter
- Lavis Lab (10) Apply Lavis Lab filter
- Lee (Albert) Lab (5) Apply Lee (Albert) Lab filter
- Li Lab (1) Apply Li Lab filter
- Lippincott-Schwartz Lab (9) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (3) Apply Liu (Zhe) Lab filter
- Looger Lab (5) Apply Looger Lab filter
- Pachitariu Lab (5) Apply Pachitariu Lab filter
- Podgorski Lab (2) Apply Podgorski Lab filter
- Reiser Lab (6) Apply Reiser Lab filter
- Romani Lab (3) Apply Romani Lab filter
- Rubin Lab (6) Apply Rubin Lab filter
- Saalfeld Lab (6) Apply Saalfeld Lab filter
- Scheffer Lab (2) Apply Scheffer Lab filter
- Schreiter Lab (6) Apply Schreiter Lab filter
- Shroff Lab (1) Apply Shroff Lab filter
- Spruston Lab (3) Apply Spruston Lab filter
- Stern Lab (4) Apply Stern Lab filter
- Sternson Lab (2) Apply Sternson Lab filter
- Stringer Lab (5) Apply Stringer Lab filter
- Svoboda Lab (5) Apply Svoboda Lab filter
- Tebo Lab (2) Apply Tebo Lab filter
- Tervo Lab (1) Apply Tervo Lab filter
- Tillberg Lab (3) Apply Tillberg Lab filter
- Truman Lab (2) Apply Truman Lab filter
- Turaga Lab (2) Apply Turaga Lab filter
- Turner Lab (11) Apply Turner Lab filter
- Vale Lab (1) Apply Vale Lab filter
- Wang (Meng) Lab (3) Apply Wang (Meng) Lab filter
- Wang (Shaohe) Lab (1) Apply Wang (Shaohe) Lab filter
Associated Project Team
- CellMap (1) Apply CellMap filter
- COSEM (1) Apply COSEM filter
- Fly Descending Interneuron (2) Apply Fly Descending Interneuron filter
- Fly Functional Connectome (2) Apply Fly Functional Connectome filter
- Fly Olympiad (1) Apply Fly Olympiad filter
- FlyEM (4) Apply FlyEM filter
- FlyLight (12) Apply FlyLight filter
- GENIE (5) Apply GENIE filter
- MouseLight (1) Apply MouseLight filter
- Tool Translation Team (T3) (3) Apply Tool Translation Team (T3) filter
Publication Date
- December 2023 (9) Apply December 2023 filter
- November 2023 (17) Apply November 2023 filter
- October 2023 (14) Apply October 2023 filter
- September 2023 (16) Apply September 2023 filter
- August 2023 (18) Apply August 2023 filter
- July 2023 (11) Apply July 2023 filter
- June 2023 (24) Apply June 2023 filter
- May 2023 (15) Apply May 2023 filter
- April 2023 (12) Apply April 2023 filter
- March 2023 (15) Apply March 2023 filter
- February 2023 (12) Apply February 2023 filter
- January 2023 (13) Apply January 2023 filter
- Remove 2023 filter 2023
Type of Publication
176 Publications
Showing 111-120 of 176 resultsAs observed in human language learning and song learning in birds, the fruit fly Drosophila melanogaster changes its' auditory behaviors according to prior sound experiences. Female flies that have heard male courtship songs of the same species are less responsive to courtship songs of different species. This phenomenon, known as song preference learning in flies, requires GABAergic input to pC1 neurons in the central brain, with these neurons playing a key role in mating behavior by integrating multimodal sensory and internal information. The neural circuit basis of this GABAergic input, however, has not yet been identified. Here, we find that pCd-2 neurons, totaling four cells per hemibrain and expressing the sex-determination gene doublesex, provide the GABAergic input to pC1 neurons for song preference learning. First, RNAi-mediated knockdown of GABA production in pCd-2 neurons abolished song preference learning. Second, pCd-2 neurons directly, and in many cases mutually, connect with pC1 neurons, suggesting the existence of reciprocal circuits between pC1 and pCd-2 neurons. Finally, GABAergic and dopaminergic inputs to pCd-2 neurons are necessary for song preference learning. Together, this study suggests that reciprocal circuits between pC1 and pCd-2 neurons serve as a sensory and internal state-integrated hub, allowing flexible control over female copulation. Consequently, this provides a neural circuit model that underlies experience-dependent auditory plasticity.
Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative, yet inadequate for understanding brain function more globally. Here, we present the first neuronal wiring diagram of a whole adult brain, containing 5×10 chemical synapses between ∼130,000 neurons reconstructed from a female . The resource also incorporates annotations of cell classes and types, nerves, hemilineages, and predictions of neurotransmitter identities. Data products are available by download, programmatic access, and interactive browsing and made interoperable with other fly data resources. We show how to derive a projectome, a map of projections between regions, from the connectome. We demonstrate the tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine, and descending neurons), across both hemispheres, and between the central brain and the optic lobes. Tracing from a subset of photoreceptors all the way to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviors. The technologies and open ecosystem of the FlyWire Consortium set the stage for future large-scale connectome projects in other species.
There is rich variety in the activity of single neurons recorded during behaviour. Yet, these diverse single neuron responses can be well described by relatively few patterns of neural co-modulation. The study of such low-dimensional structure of neural population activity has provided important insights into how the brain generates behaviour. Virtually all of these studies have used linear dimensionality reduction techniques to estimate these population-wide co-modulation patterns, constraining them to a flat "neural manifold". Here, we hypothesised that since neurons have nonlinear responses and make thousands of distributed and recurrent connections that likely amplify such nonlinearities, neural manifolds should be intrinsically nonlinear. Combining neural population recordings from monkey motor cortex, mouse motor cortex, mouse striatum, and human motor cortex, we show that: 1) neural manifolds are intrinsically nonlinear; 2) the degree of their nonlinearity varies across architecturally distinct brain regions; and 3) manifold nonlinearity becomes more evident during complex tasks that require more varied activity patterns. Simulations using recurrent neural network models confirmed the proposed relationship between circuit connectivity and manifold nonlinearity, including the differences across architecturally distinct regions. Thus, neural manifolds underlying the generation of behaviour are inherently nonlinear, and properly accounting for such nonlinearities will be critical as neuroscientists move towards studying numerous brain regions involved in increasingly complex and naturalistic behaviours.
Activity related to movement is found throughout sensory and motor regions of the brain. However, it remains unclear how movement-related activity is distributed across the brain and whether systematic differences exist between brain areas. Here, we analyzed movement related activity in brain-wide recordings containing more than 50,000 neurons in mice performing a decision-making task. Using multiple techniques, from markers to deep neural networks, we find that movement-related signals were pervasive across the brain, but systematically differed across areas. Movement-related activity was stronger in areas closer to the motor or sensory periphery. Delineating activity in terms of sensory- and motor-related components revealed finer scale structures of their encodings within brain areas. We further identified activity modulation that correlates with decision-making and uninstructed movement. Our work charts out a largescale map of movement encoding and provides a roadmap for dissecting different forms of movement and decision-making related encoding across multi-regional neural circuits.
A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the Open Microscopy Environment (OME), individuals and institutes across diverse modalities facing these problems have designed a format specification process (OME-NGFF) to address these needs. This paper brings together a wide range of those community members to describe the format itself – OME-Zarr – along with tools and data resources available today to increase FAIR access and remove barriers in the scientific process. The current momentum offers an opportunity to unify a key component of the bioimaging domain — the file format that underlies so many personal, institutional, and global data management and analysis tasks.
Rhodamine dyes are excellent scaffolds for developing a broad range of fluorescent probes. A key property of rhodamines is their equilibrium between a colorless lactone and fluorescent zwitterion. Tuning the lactone–zwitterion equilibrium constant (KL–Z) can optimize dye properties for specific biological applications. Here, we use known and novel organic chemistry to prepare a comprehensive collection of rhodamine dyes to elucidate the structure–activity relationships that govern KL–Z. We discovered that the auxochrome substituent strongly affects the lactone–zwitterion equilibrium, providing a roadmap for the rational design of improved rhodamine dyes. Electron-donating auxochromes, such as julolidine, work in tandem with fluorinated pendant phenyl rings to yield bright, red-shifted fluorophores for live-cell single-particle tracking (SPT) and multicolor imaging. The N-aryl auxochrome combined with fluorination yields red-shifted Förster resonance energy transfer (FRET) quencher dyes useful for creating a new semisynthetic indicator to sense cAMP using fluorescence lifetime imaging microscopy (FLIM). Together, this work expands the synthetic methods available for rhodamine synthesis, generates new reagents for advanced fluorescence imaging experiments, and describes structure–activity relationships that will guide the design of future probes.
Natural behaviors are a coordinated symphony of motor acts which drive self-induced or reafferent sensory activation. Single sensors only signal presence and magnitude of a sensory cue; they cannot disambiguate exafferent (externally-induced) from reafferent sources. Nevertheless, animals readily differentiate between these sources of sensory signals to make appropriate decisions and initiate adaptive behavioral outcomes. This is mediated by predictive motor signaling mechanisms, which emanate from motor control pathways to sensory processing pathways, but how predictive motor signaling circuits function at the cellular and synaptic level is poorly understood. We use a variety of techniques, including connectomics from both male and female electron microscopy volumes, transcriptomics, neuroanatomical, physiological and behavioral approaches to resolve the network architecture of two pairs of ascending histaminergic neurons (AHNs), which putatively provide predictive motor signals to several sensory and motor neuropil. Both AHN pairs receive input primarily from an overlapping population of descending neurons, many of which drive wing motor output. The two AHN pairs target almost exclusively non-overlapping downstream neural networks including those that process visual, auditory and mechanosensory information as well as networks coordinating wing, haltere, and leg motor output. These results support the conclusion that the AHN pairs multi-task, integrating a large amount of common input, then tile their output in the brain, providing predictive motor signals to non-overlapping sensory networks affecting motor control both directly and indirectly.
Our ability to remember the past is essential for guiding our future behavior. Psychological and neurobiological features of declarative memories are known to transform over time in a process known as systems consolidation. While many theories have sought to explain the time-varying role of hippocampal and neocortical brain areas, the computational principles that govern these transformations remain unclear. Here we propose a theory of systems consolidation in which hippocampal-cortical interactions serve to optimize generalizations that guide future adaptive behavior. We use mathematical analysis of neural network models to characterize fundamental performance tradeoffs in systems consolidation, revealing that memory components should be organized according to their predictability. The theory shows that multiple interacting memory systems can outperform just one, normatively unifying diverse experimental observations and making novel experimental predictions. Our results suggest that the psychological taxonomy and neurobiological organization of declarative memories reflect a system optimized for behaving well in an uncertain future.
How does wiring specificity of neural maps emerge during development? Formation of the adult olfactory glomerular map begins with patterning of projection neuron (PN) dendrites at the early pupal stage. To better understand the origin of wiring specificity of this map, we created genetic tools to systematically characterize dendrite patterning across development at PN type-specific resolution. We find that PNs use lineage and birth order combinatorially to build the initial dendritic map. Specifically, birth order directs dendrite targeting in rotating and binary manners for PNs of the anterodorsal and lateral lineages, respectively. Two-photon- and adaptive optical lattice light-sheet microscope-based time-lapse imaging reveals that PN dendrites initiate active targeting with direction-dependent branch stabilization on the timescale of seconds. Moreover, PNs that are used in both the larval and adult olfactory circuits prune their larval-specific dendrites and re-extend new dendrites simultaneously to facilitate timely olfactory map organization. Our work highlights the power and necessity of type-specific neuronal access and time-lapse imaging in identifying wiring mechanisms that underlie complex patterns of functional neural maps.
Proteins localized at the cellular interface mediate cell-cell communication and thus control many aspects of physiology in multicellular organisms. Cell-surface proteomics allows biologists to comprehensively identify proteins on the cell surface and survey their dynamics in physiological and pathological conditions. PEELing provides an integrated package and user-centric web service for analyzing cell-surface proteomics data. With a streamlined and automated workflow, PEELing evaluates data quality using curated references, performs cutoff analysis to remove contaminants, connects to databases for functional annotation, and generates data visualizations. Together with chemical and transgenic tools, PEELing completes a pipeline making cell-surface proteomics analysis handy for every lab.