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What We Do
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SERVICES
Primary culture
- Oganotypic (brain slice) cultures
- Dissection and culture of different brain regions including (but not limited to) cortical, hippocampal, midbrain.
- Dorsal root ganglia dissection and culture
- Mouse embryonic feeder production
- Hepatocyte primary cultures
- Cell transfection using electroporation and viral transduction
- Drosophila neuronal cultures
- We can develop new protocols according to the request
Pluripotent Stem cells (PSC)
- Cell banking
- Quality control including pluripotency test, chromosomal abnormalities, mycoplasma test
- Cell transfection using electroporation and viral transduction
- Gene editing of primary and Pluripotent Stem cells using CRISPR, transposon or lipid-based systems
- Maintaining and expanding both iPSC and ESC
- Differentiation of PSCs into neuronal, cardiac, liver, and other cell types
- Differentiation of PSC into organoids i.e., cerebral organoids
- Hydrogels
- Microfluidics
EQUIPMENT
- Laminar flow and biosafety cabinets
- Fluorescent dissecting microscopes
- Zeiss fluorescent dissecting microscope equipped to handle biohazardous materials
- Evos live cell imaging microscope
- Lonza 4D nucleofector
- CO2 and Oxygen (trigas) controlled incubators
- Cell Vivo organoid incubator
- Rotational incubator
- Primo system micropatterning
- Leica epifluorescent microscope
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