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What We Do
The Primary and iPS Cell Culture Team provides expertise in the creation of primary cells and the maintenance and differentiation of iPSCs.
Primary cells are created from tissues collected from a variety of organisms, including drosophila, rodent, and fish. We can customize other needs. We also offer organotypic cultures from different brain regions.
Work with Pluripotent Stem Cells (PSC) is focused on maintaining, genome editing, and differentiating into many cell types, including (but not restricted to) neurons of different phenotypes, hepatocytes, and cardiomyocytes. We can also differentiate into organoids and customize cultures into microfluidic devices that in collaboration with other teams can be custom made.
Our team members are specialized in all different areas of cell culture, stem cell biology and bioengineering. We will brainstorm ideas to design experiments that will better fit your project and answer questions more readily.
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What We Offer
- Laminar flow and biosafety cabinets, fluorescent dissecting microscopes, EVOS live cell imaging microscope, Lonza 4D Electroporator, CO2 and tri-gas incubators, organoid incubators, micropatterning, hydrogels, and other necessary equipment for the collection of tissue and maintenance of primary and iPS cells by the shared resource or researchers.
- Quality control of equipment and reagents.
- Cell transfection using electroporation and viral transduction.
- Gene editing of primary and iPS cells using CRISPR, transposon or any other system.
- Maintaining iPSCs to ensure pluripotency.
- Differentiation of iPSCs into neuronal, cardiac, and liver and other cell types.
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