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- Anatomy and Histology
- Cryo-Electron Microscopy
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janelia7_blocks-janelia7_biblio_header | block
Journal of Biomolecular Techniques. 2020 Aug;31(Suppl):S26
A cytometry-based assay to determine optimal Janelia Fluor dye labelling of Halo and SNAP tags.
Schaefer K, Xie FL, Weis-Garcia F, White N
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Abstract
When used in combination, self-labelling protein tags such as Halo, SNAP, and CLIP allow for the simultaneous visualization of proteins across a wide fluorescence spectrum. However, the combination of cell type, ligand binding and fluorescent dye chemistry introduces several variables that need to be determined to achieve orthogonal labelling. The Janelia Cell Culture Shared Resource in collaboration with a Research Scientist, and the Lavis Lab have developed a high throughput cytometry-based assay to determine optimal conditions for various combinations of cell type, ligand and JF dyes.
PMID: 32831738 [PubMed - indexed for MEDLINE]
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janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
Liangqi Xie
janelia7_blocks-janelia7_biblio_tools | block