A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions.

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bioRxiv. 2024 Feb 16;. doi: 10.1101/2024.02.15.580492

Farrell Ryan, Bredvik Kirsten, Hoppa Michael, Hennigan Thomas, Brown Timothy, Ryan Timothy

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Abstract

The endoplasmic reticulum (ER) is an important regulator of Ca2+ in cells and dysregulation of ER calcium homeostasis can lead to numerous pathologies. Understanding how various pharmacological and genetic perturbations of ER Ca2+ homeostasis impacts cellular physiology would likely be facilitated by more quantitative measurements of ER Ca2+ levels that allow easier comparisons across conditions. Here, we developed a ratiometric version of our original ER-GCaMP probe that allows for more quantitative comparisons of the concentration of Ca2+ in the ER across cell types and sub-cellular compartments. Using this approach we show that the resting concentration of ER Ca2+ in primary dissociated neurons is substantially lower than that in measured in embryonic fibroblasts.

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bioRxiv Preprint https://doi.org/10.1101/2024.02.15.580492

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A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions.

Abstract

Janelia Authors