BrainAligner: 3D registration atlases of Drosophila brains.

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Nature Methods. 2011 Jun;8:493-500. doi: 10.1038/nmeth.1602

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BrainAligner: 3D registration atlases of Drosophila brains. Simpson LabRubin Lab

Peng H, Chung P, Long F, Qu L, Jenett A, Seeds AM, Myers EW, Simpson JH

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Abstract

Analyzing Drosophila melanogaster neural expression patterns in thousands of three-dimensional image stacks of individual brains requires registering them into a canonical framework based on a fiducial reference of neuropil morphology. Given a target brain labeled with predefined landmarks, the BrainAligner program automatically finds the corresponding landmarks in a subject brain and maps it to the coordinate system of the target brain via a deformable warp. Using a neuropil marker (the antibody nc82) as a reference of the brain morphology and a target brain that is itself a statistical average of data for 295 brains, we achieved a registration accuracy of 2 μm on average, permitting assessment of stereotypy, potential connectivity and functional mapping of the adult fruit fly brain. We used BrainAligner to generate an image pattern atlas of 2954 registered brains containing 470 different expression patterns that cover all the major compartments of the fly brain.

PMID: 21532582 [PubMed - indexed for MEDLINE]

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BrainAligner: 3D registration atlases of Drosophila brains.

Abstract

PMID: 21532582 [PubMed - indexed for MEDLINE]

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