Imaging light responses of targeted neuron populations in the rodent retina.

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The Journal of Neuroscience: The Official Journal of the Society for Neuroscience. 2011 Feb 23;31:2855-67. doi: 10.1523/JNEUROSCI.6064-10.2011

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Imaging light responses of targeted neuron populations in the rodent retina. Looger LabLeonardo Lab

Borghuis BG, Tian L, Xu Y, Nikonov SS, Vardi N, Zemelman BV, Looger LL

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Abstract

Decoding the wiring diagram of the retina requires simultaneous observation of activity in identified neuron populations. Available recording methods are limited in their scope: electrodes can access only a small fraction of neurons at once, whereas synthetic fluorescent indicator dyes label tissue indiscriminately. Here, we describe a method for studying retinal circuitry at cellular and subcellular levels combining two-photon microscopy and a genetically encoded calcium indicator. Using specific viral and promoter constructs to drive expression of GCaMP3, we labeled all five major neuron classes in the adult mouse retina. Stimulus-evoked GCaMP3 responses as imaged by two-photon microscopy permitted functional cell type annotation. Fluorescence responses were similar to those measured with the small molecule dye OGB-1. Fluorescence intensity correlated linearly with spike rates >10 spikes/s, and a significant change in fluorescence always reflected a significant change in spike firing rate. GCaMP3 expression had no apparent effect on neuronal function. Imaging at subcellular resolution showed compartment-specific calcium dynamics in multiple identified cell types.

PMID: 21414907 [PubMed - indexed for MEDLINE]

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Imaging light responses of targeted neuron populations in the rodent retina.

Abstract

PMID: 21414907 [PubMed - indexed for MEDLINE]

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