Filter
Associated Lab
- Aguilera Castrejon Lab (1) Apply Aguilera Castrejon Lab filter
- Ahrens Lab (4) Apply Ahrens Lab filter
- Aso Lab (6) Apply Aso Lab filter
- Betzig Lab (4) Apply Betzig Lab filter
- Branson Lab (2) Apply Branson Lab filter
- Card Lab (6) Apply Card Lab filter
- Clapham Lab (1) Apply Clapham Lab filter
- Darshan Lab (4) Apply Darshan Lab filter
- Dickson Lab (4) Apply Dickson Lab filter
- Dudman Lab (2) Apply Dudman Lab filter
- Espinosa Medina Lab (1) Apply Espinosa Medina Lab filter
- Feliciano Lab (1) Apply Feliciano Lab filter
- Fitzgerald Lab (5) Apply Fitzgerald Lab filter
- Funke Lab (12) Apply Funke Lab filter
- Harris Lab (7) Apply Harris Lab filter
- Hermundstad Lab (2) Apply Hermundstad Lab filter
- Hess Lab (6) Apply Hess Lab filter
- Jayaraman Lab (1) Apply Jayaraman Lab filter
- Karpova Lab (2) Apply Karpova Lab filter
- Keller Lab (1) Apply Keller Lab filter
- Lavis Lab (10) Apply Lavis Lab filter
- Lee (Albert) Lab (5) Apply Lee (Albert) Lab filter
- Li Lab (1) Apply Li Lab filter
- Lippincott-Schwartz Lab (9) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (3) Apply Liu (Zhe) Lab filter
- Looger Lab (5) Apply Looger Lab filter
- Pachitariu Lab (5) Apply Pachitariu Lab filter
- Podgorski Lab (2) Apply Podgorski Lab filter
- Reiser Lab (6) Apply Reiser Lab filter
- Romani Lab (3) Apply Romani Lab filter
- Rubin Lab (6) Apply Rubin Lab filter
- Saalfeld Lab (6) Apply Saalfeld Lab filter
- Scheffer Lab (2) Apply Scheffer Lab filter
- Schreiter Lab (6) Apply Schreiter Lab filter
- Shroff Lab (1) Apply Shroff Lab filter
- Spruston Lab (3) Apply Spruston Lab filter
- Stern Lab (4) Apply Stern Lab filter
- Sternson Lab (2) Apply Sternson Lab filter
- Stringer Lab (5) Apply Stringer Lab filter
- Svoboda Lab (5) Apply Svoboda Lab filter
- Tebo Lab (2) Apply Tebo Lab filter
- Tervo Lab (1) Apply Tervo Lab filter
- Tillberg Lab (3) Apply Tillberg Lab filter
- Truman Lab (2) Apply Truman Lab filter
- Turaga Lab (2) Apply Turaga Lab filter
- Turner Lab (11) Apply Turner Lab filter
- Vale Lab (1) Apply Vale Lab filter
- Wang (Meng) Lab (3) Apply Wang (Meng) Lab filter
- Wang (Shaohe) Lab (1) Apply Wang (Shaohe) Lab filter
Associated Project Team
- CellMap (1) Apply CellMap filter
- COSEM (1) Apply COSEM filter
- Fly Descending Interneuron (2) Apply Fly Descending Interneuron filter
- Fly Functional Connectome (2) Apply Fly Functional Connectome filter
- Fly Olympiad (1) Apply Fly Olympiad filter
- FlyEM (4) Apply FlyEM filter
- FlyLight (12) Apply FlyLight filter
- GENIE (5) Apply GENIE filter
- MouseLight (1) Apply MouseLight filter
- Tool Translation Team (T3) (3) Apply Tool Translation Team (T3) filter
Publication Date
- December 2023 (9) Apply December 2023 filter
- November 2023 (17) Apply November 2023 filter
- October 2023 (14) Apply October 2023 filter
- September 2023 (16) Apply September 2023 filter
- August 2023 (18) Apply August 2023 filter
- July 2023 (11) Apply July 2023 filter
- June 2023 (24) Apply June 2023 filter
- May 2023 (15) Apply May 2023 filter
- April 2023 (12) Apply April 2023 filter
- March 2023 (15) Apply March 2023 filter
- February 2023 (12) Apply February 2023 filter
- January 2023 (13) Apply January 2023 filter
- Remove 2023 filter 2023
Type of Publication
176 Publications
Showing 91-100 of 176 resultsA tool to map changes in synaptic strength during a defined time window could provide powerful insights into the mechanisms governing learning and memory. We developed a technique, Extracellular Protein Surface Labeling in Neurons (EPSILON), to map α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) insertion in vivo by pulse-chase labeling of surface AMPARs with membrane-impermeable dyes. This approach allows for single-synapse resolution maps of plasticity in genetically targeted neurons during memory formation. We investigated the relationship between synapse-level and cell-level memory encodings by mapping synaptic plasticity and cFos expression in hippocampal CA1 pyramidal cells upon contextual fear conditioning (CFC). We observed a strong correlation between synaptic plasticity and cFos expression, suggesting a synaptic mechanism for the association of cFos expression with memory engrams. The EPSILON technique is a useful tool for mapping synaptic plasticity and may be extended to investigate trafficking of other transmembrane proteins.
Hippocampal area CA3 is thought to play a central role in memory formation and retrieval. Although various network mechanisms have been hypothesized to mediate these computations, direct evidence is lacking. Using intracellular membrane potential recordings from CA3 neurons and optogenetic manipulations in behaving mice we found that place field activity is produced by a symmetric form of Behavioral Timescale Synaptic Plasticity (BTSP) at recurrent synaptic connections among CA3 principal neurons but not at synapses from the dentate gyrus (DG). Additional manipulations revealed that excitatory input from the entorhinal cortex (EC) but not DG was required to update place cell activity based on the animal’s movement. These data were captured by a computational model that used BTSP and an external updating input to produce attractor dynamics under online learning conditions. Additional theoretical results demonstrate the enhanced memory storage capacity of such networks, particularly in the face of correlated input patterns. The evidence sheds light on the cellular and circuit mechanisms of learning and memory formation in the hippocampus.
The hippocampus is critical for recollecting and imagining experiences. This is believed to involve voluntarily drawing from hippocampal memory representations of people, events, and places, including the hippocampus’ map-like representations of familiar environments. However, whether the representations in such “cognitive maps” can be volitionally and selectively accessed is unknown. We developed a brain-machine interface to test if rats could control their hippocampal activity in a flexible, goal-directed, model-based manner. We show that rats can efficiently navigate or direct objects to arbitrary goal locations within a virtual reality arena solely by activating and sustaining appropriate hippocampal representations of remote places. This should provide insight into the mechanisms underlying episodic memory recall, mental simulation/planning, and imagination, and open up possibilities for high-level neural prosthetics utilizing hippocampal representations.
Recent success in training artificial agents and robots derives from a combination of direct learning of behavioural policies and indirect learning through value functions. Policy learning and value learning use distinct algorithms that optimize behavioural performance and reward prediction, respectively. In animals, behavioural learning and the role of mesolimbic dopamine signalling have been extensively evaluated with respect to reward prediction; however, so far there has been little consideration of how direct policy learning might inform our understanding. Here we used a comprehensive dataset of orofacial and body movements to understand how behavioural policies evolved as naive, head-restrained mice learned a trace conditioning paradigm. Individual differences in initial dopaminergic reward responses correlated with the emergence of learned behavioural policy, but not the emergence of putative value encoding for a predictive cue. Likewise, physiologically calibrated manipulations of mesolimbic dopamine produced several effects inconsistent with value learning but predicted by a neural-network-based model that used dopamine signals to set an adaptive rate, not an error signal, for behavioural policy learning. This work provides strong evidence that phasic dopamine activity can regulate direct learning of behavioural policies, expanding the explanatory power of reinforcement learning models for animal learning.
Animals can learn general task structures and use them to solve new problems with novel sensory specifics. This capacity of ‘learning to learn’, or meta-learning, is difficult to achieve in artificial systems, and the mechanisms by which it is achieved in animals are unknown. As a step toward enabling mechanistic studies, we developed a behavioral paradigm that demonstrates meta-learning in head-fixed mice. We trained mice to perform a two-alternative forced-choice task in virtual reality (VR), and successively changed the visual cues that signaled reward location. Mice showed increased learning speed in both cue generalization and serial reversal tasks. During reversal learning, behavior exhibited sharp transitions, with the transition occurring earlier in each successive reversal. Analysis of motor patterns revealed that animals utilized similar motor programs to execute the same actions in response to different cues but modified the motor programs during reversal learning. Our study demonstrates that mice can perform meta-learning tasks in VR, thus opening up opportunities for future mechanistic studies.
Insects like Drosophila produce a second brain adapted to the form and behavior of a larva. Neurons for both larval and adult brains are produced by the same stem cells (neuroblasts) but the larva possesses only the earliest born neurons produced from each. To understand how a functional larval brain is made from this reduced set of neurons, we examined the origins and metamorphic fates of the neurons of the larval and adult mushroom body circuits. The adult mushroom body core is built sequentially of γ Kenyon cells, that form a medial lobe, followed by α’β’, and αβ Kenyon cells that form additional medial lobes and two vertical lobes. Extrinsic input (MBINs) and output (MBONs) neurons divide this core into computational compartments. The larval mushroom body contains only γ neurons. Its medial lobe compartments are roughly homologous to those of the adult and same MBONs are used for both. The larval vertical lobe, however, is an analogous “facsimile” that uses a larval-specific branch on the γ neurons to make up for the missing α’β’, and αβ neurons. The extrinsic cells for the facsimile are early-born neurons that trans-differentiate to serve a mushroom body function in the larva and then shift to other brain circuits in the adult. These findings are discussed in the context of the evolution of a larval brain in insects with complete metamorphosis.
Heptamethine indocyanines are invaluable probes for near-infrared (NIR) imaging. Despite broad use, there are only a few synthetic methods to assemble these molecules, and each has significant limitations. Here, we report the use of pyridinium benzoxazole (PyBox) salts as heptamethine indocyanine precursors. This method is high yielding, simple to implement, and provides access to previously unknown chromophore functionality. We applied this method to create molecules to address two outstanding objectives in NIR fluorescence imaging. First, we used an iterative approach to develop molecules for protein-targeted tumor imaging. When compared to common NIR fluorophores, the optimized probe increases the tumor specificity of monoclonal antibody (mAb) and nanobody conjugates. Second, we developed cyclizing heptamethine indocyanines with the goal of improving cellular uptake and fluorogenic properties. By modifying both the electrophilic and nucleophilic components, we demonstrate that the solvent sensitivity of the ring-open/ring-closed equilibrium can be modified over a wide range. We then show that a chloroalkane derivative of a compound with tuned cyclization properties undergoes particularly efficient no-wash live cell imaging using organelle-targeted HaloTag self-labeling proteins. Overall, the chemistry reported here broadens the scope of accessible chromophore functionality, and, in turn, enables the discovery of NIR probes with promising properties for advanced imaging applications.
Healthy mitochondria are critical for reproduction. During aging, both reproductive fitness and mitochondrial homeostasis decline. Mitochondrial metabolism and dynamics are key factors in supporting mitochondrial homeostasis. However, how they are coupled to control reproductive health remains unclear. We report that mitochondrial GTP (mtGTP) metabolism acts through mitochondrial dynamics factors to regulate reproductive aging. We discovered that germline-only inactivation of GTP- but not ATP-specific succinyl-CoA synthetase (SCS) promotes reproductive longevity in Caenorhabditis elegans. We further identified an age-associated increase in mitochondrial clustering surrounding oocyte nuclei, which is attenuated by GTP-specific SCS inactivation. Germline-only induction of mitochondrial fission factors sufficiently promotes mitochondrial dispersion and reproductive longevity. Moreover, we discovered that bacterial inputs affect mtGTP levels and dynamics factors to modulate reproductive aging. These results demonstrate the significance of mtGTP metabolism in regulating oocyte mitochondrial homeostasis and reproductive longevity and identify mitochondrial fission induction as an effective strategy to improve reproductive health.
Healthy mitochondria are critical for reproduction. During aging, both reproductive fitness and mitochondrial homeostasis decline. Mitochondrial metabolism and dynamics are key factors in supporting mitochondrial homeostasis. However, how they are coupled to control reproductive health remains unclear. We report that mitochondrial GTP (mtGTP) metabolism acts through mitochondrial dynamics factors to regulate reproductive aging. We discovered that germline-only inactivation of GTP- but not ATP-specific succinyl-CoA synthetase (SCS) promotes reproductive longevity in Caenorhabditis elegans. We further identified an age-associated increase in mitochondrial clustering surrounding oocyte nuclei, which is attenuated by GTP-specific SCS inactivation. Germline-only induction of mitochondrial fission factors sufficiently promotes mitochondrial dispersion and reproductive longevity. Moreover, we discovered that bacterial inputs affect mtGTP levels and dynamics factors to modulate reproductive aging. These results demonstrate the significance of mtGTP metabolism in regulating oocyte mitochondrial homeostasis and reproductive longevity and identify mitochondrial fission induction as an effective strategy to improve reproductive health.
Healthy mitochondria are critical for reproduction. During aging, both reproductive fitness and mitochondrial homeostasis decline. Mitochondrial metabolism and dynamics are key factors in supporting mitochondrial homeostasis. However, how they are coupled to control reproductive health remains unclear. We report that mitochondrial GTP metabolism acts through mitochondrial dynamics factors to regulate reproductive aging. We discovered that germline-only inactivation of GTP- but not ATP-specific succinyl-CoA synthetase (SCS), promotes reproductive longevity in Caenorhabditis elegans. We further revealed an age-associated increase in mitochondrial clustering surrounding oocyte nuclei, which is attenuated by the GTP-specific SCS inactivation. Germline-only induction of mitochondrial fission factors sufficiently promotes mitochondrial dispersion and reproductive longevity. Moreover, we discovered that bacterial inputs affect mitochondrial GTP and dynamics factors to modulate reproductive aging. These results demonstrate the significance of mitochondrial GTP metabolism in regulating oocyte mitochondrial homeostasis and reproductive longevity and reveal mitochondrial fission induction as an effective strategy to improve reproductive health.