Preparation of cuticles from unhatched first-instar Drosophila larvae.

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Cold Spring Harb Protoc. 2011 Sep;2011(9). doi: 10.1101/pdb.prot065532

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Preparation of cuticles from unhatched first-instar Drosophila larvae. Stern Lab

Stern DL, Sucena E

Note: Research in this publication was not performed at Janelia.

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Abstract

The finely sculpted cuticle of Drosophila carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. Studies of the Drosophila cuticle have focused mainly on first-instar larvae and adult cuticular morphology. This protocol describes the preparation of cuticles from larvae that have not yet hatched from the egg. It is designed for sampling all eggs laid by one or more females. This can be particularly useful, for example, when a mutation produces embryos that are unable to hatch from the egg.

PMID: 21880820 [PubMed - indexed for MEDLINE]

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Preparation of cuticles from unhatched first-instar Drosophila larvae.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 21880820 [PubMed - indexed for MEDLINE]

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