Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.

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Nature Methods. 2014 Dec;11(12):1237-41. doi: 10.1038/nmeth.3146

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Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology. Harris Lab

Andrasfalvy BK, Galiñanes GL, Huber D, Barbic M, Macklin JJ, Susumu K, Delehanty JB, Huston AL, Makara JK, Medintz IL

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Abstract

Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.

PMID: 25326662 [PubMed - indexed for MEDLINE]

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Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.

Abstract

PMID: 25326662 [PubMed - indexed for MEDLINE]

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