Direct observation of frequency modulated transcription in single cells using light activation.

Our Research / Publications

janelia7_blocks-janelia7_biblio_header | block

eLife. 2013 Sep 24;2:e00750. doi: 10.7554/eLife.00750

View Article on

Direct observation of frequency modulated transcription in single cells using light activation. Singer Lab

Larson DR, Fritzsch C, Sun L, Meng X, Lawrence DS, Singer RH

Note: Research in this publication was not performed at Janelia.

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus. DOI:http://dx.doi.org/10.7554/eLife.00750.001.

PMID: 24069527 [PubMed - indexed for MEDLINE]

node:body | entity_field

Main Menu (Mobile)- Block

Main Menu - Block

Direct observation of frequency modulated transcription in single cells using light activation.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 24069527 [PubMed - indexed for MEDLINE]

Janelia Authors