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janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305
Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Singer Lab
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Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
Note: Research in this publication was not performed at Janelia.
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.
PMID: 23263691 [PubMed - indexed for MEDLINE]
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janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
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