GFP-aequorin protein sensor for ex vivo and in vivo imaging of Ca(2+) dynamics in high-Ca(2+) organelles.

Our Research / Publications

janelia7_blocks-janelia7_biblio_header | block

Cell Chemical Biology. 2016 Jun 1;. doi: 10.1016/j.chembiol.2016.05.010

View Article on

GFP-aequorin protein sensor for ex vivo and in vivo imaging of Ca(2+) dynamics in high-Ca(2+) organelles. Looger Lab

Navas-Navarro P, Rojo-Ruiz J, Rodriguez-Prados M, Ganfornina MD, Looger LL, Alonso MT, García-Sancho J

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Proper functioning of organelles such as the ER or the Golgi apparatus requires luminal accumulation of Ca(2+) at high concentrations. Here we describe a ratiometric low-affinity Ca(2+) sensor of the GFP-aequorin protein (GAP) family optimized for measurements in high-Ca(2+) concentration environments. Transgenic animals expressing the ER-targeted sensor allowed monitoring of Ca(2+) signals inside the organelle. The use of the sensor was demonstrated under three experimental paradigms: (1) ER Ca(2+) oscillations in cultured astrocytes, (2) ex vivo functional mapping of cholinergic receptors triggering ER Ca(2+) release in acute hippocampal slices from transgenic mice, and (3) in vivo sarcoplasmic reticulum Ca(2+) dynamics in the muscle of transgenic flies. Our results provide proof of the suitability of the new biosensors to monitor Ca(2+) dynamics inside intracellular organelles under physiological conditions and open an avenue to explore complex Ca(2+) signaling in animal models of health and disease.

PMID: 27291400 [PubMed - indexed for MEDLINE]

node:body | entity_field

Main Menu (Mobile)- Block

Main Menu - Block

GFP-aequorin protein sensor for ex vivo and in vivo imaging of Ca(2+) dynamics in high-Ca(2+) organelles.

Abstract

PMID: 27291400 [PubMed - indexed for MEDLINE]

Janelia Authors