Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2.

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Nature Structural & Molecular Biology. 2018 Mar 12;. doi: 10.1038/s41594-018-0045-5

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Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2. Gonen Lab

Guenther EL, Ge P, Trinh H, Sawaya MR, Cascio D, Boyer DR, Gonen T, Zhou ZH, Eisenberg DS

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Abstract

Proteins in the fibrous amyloid state are a major hallmark of neurodegenerative disease. Understanding the multiple conformations, or polymorphs, of amyloid proteins at the molecular level is a challenge of amyloid research. Here, we detail the wide range of polymorphs formed by a segment of human TAR DNA-binding protein 43 (TDP-43) as a model for the polymorphic capabilities of pathological amyloid aggregation. Using X-ray diffraction, microelectron diffraction (MicroED) and single-particle cryo-EM, we show that theDLIIKGISVHIsegment from the second RNA-recognition motif (RRM2) forms an array of amyloid polymorphs. These associations include seven distinct interfaces displaying five different symmetry classes of steric zippers. Additionally, we find that this segment can adopt three different backbone conformations that contribute to its polymorphic capabilities. The polymorphic nature of this segment illustrates at the molecular level how amyloid proteins can form diverse fibril structures.

PMID: 29531287 [PubMed - indexed for MEDLINE]

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Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2.

Abstract

PMID: 29531287 [PubMed - indexed for MEDLINE]

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