Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors.

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ACS Chemical Biology. 2018 Jul;13:2392 – 2397. doi: 10.1021/acschembio.8b00417

Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors. Tebo Lab

Tebo AG, Pimenta FM, Zoumpoulaki M, Kikuti C, Sirkia H, Plamont M, Houdusse A, Gautier A

Note: Research in this publication was not performed at Janelia.

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Abstract

Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we describe the development of circularly permuted variants of Fluorescence-Activating and absorption-Shifting Tag (FAST) and demonstrate their potential as reporting module in biosensors. Circularly permutated FAST (cpFAST) variants allow one to condition the binding and activation of a fluorogenic ligand (and thus fluorescence) to analyte recognition by coupling them with analyte-binding domains. We demonstrated their use for biosensor design by generating multicolor plug-and-play fluorogenic biosensors for imaging the intracellular levels of Ca2+ in living mammalian cells in real time.

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Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors.

Note: Research in this publication was not performed at Janelia.

Abstract

Janelia Authors