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QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

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Journal of Microscopy. 2021 Oct 01;284(1):56-73
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QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy
Nelson Glyn, Boehm Ulrike, Bagley Steve, Bajcsy Peter, Bischof Johanna, Brown Claire, Dauphin Aurelien, Dobbie Ian, Eriksson John, Faklaris Orestis, Fernandez-Rodriguez Julia, Ferrand Alexia, Gheisari Ali, Hartmann Hella, Kukat Christian, Laude Alex, Mitkovski Miso, Munck Sebastian, North Alison, Rasse Tobias, Resch-Genger Ute, Schuetz Lucas, Seitz Arne, Strambio-De-Castillia Caterina, Swedlow Jason, Alexopoulos Ioannis, Aumayr Karin, Avilov Sergiy, Bakker Gert-Jan, Bammann Rodrigo, Bassi Andrea, Beckert Hannes, Beer Sebastian, Belyaev Yury, Bierwagen Jakob, Birngruber Konstantin, Bosch Manel, Breitlow Juergen, Cameron Lisa, Chalfoun Joe, Chambers James, Chen Chieh-Li, Conde-Sousa Eduardo, Corbett Alexander, Cordelieres Fabrice, Del Nery Elaine, Dietzel Ralf, Eismann Frank, Fazeli Elnaz, Felscher Andreas, Fried Hans, Gaudreault Nathalie, Goh Wah, Guilbert Thomas, Hadleigh Roland, Hemmerich Peter, Holst Gerhard, Itano Michelle, Jaffe Claudia, Jambor Helena, Jarvis Stuart, Keppler Antje, Kirchenbuechler David, Kirchner Marcel, Kobayashi Norio, Krens Gabriel, Kunis Susanne, Lacoste Judith, Marcell Marco, Martins Gabriel, Metcalf Daniel, Mitchell Claire, Moore Joshua, Mueller Tobias, Nelson Michael, Ogg Stephen, Onami Shuichi, Palmer Alexandra, Paul-Gilloteaux Perrine, Pimentel Jaime, Plantard Laure, Podder Santosh, Rexhepaj Elton, Royon Arnaud, Saari Markku, Schapman Damien, Schoonderwoert Vincent, Schroth-Diez Britta, Schwartz Stanley, Shaw Michael, Spitaler Martin, Stoeckl Martin, Sudar Damir, Teillon Jeremie, Terjung Stefan, Thuenauer Roland, Wilms Christian, Wright Graham, Nitschke Roland, Gelman Laurent
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Abstract

In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.

PMID: 34214188 [PubMed - indexed for MEDLINE]

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Previous arXiv PrePrint https://arxiv.org/abs/2101.09153

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Janelia Authors

Ulrike Boehm
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