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Flow Cytometry

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Support Teams / Flow Cytometry
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Mission Statement

Flow Cytometry supports the Janelia scientific research community by providing training, instrumentation, and expertise to perform state-of-the-art flow cytometry analysis and cell sorting.

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Flow Cytometry Instruments

CytoFLEX S

  • The Cytoflex S is a flow cytometer used for fluorescence signal analysis; it does not perform sorting.

  • Four lasers

    • 405nm Violet, 488nm Blue, 561nm Yellow Green, 638nm Red

  • 13 fluorescence channel detectors

  • Light scatter detection off the Violet laser for small particle/exosome analysis

  • Samples can be loaded from traditional 12x75mm flow tubes or 96-well plates.

 

Sony SH800

  • Sony SH800 is a two-way cell sorter. 

  • Three nozzle sizes available:

    • 70um, 100um, 130um

  •  Four colinear lasers:

    • 405nm, 488nm, 561nm, 638nm (single interrogation site)

  • 6 fluorescence detection channels

 

Bigfoot 9 Laser Spectral Sorter

  • The Bigfoot is a six-way cell sorter.

  • Four nozzle sizes available:

    •  70um, 100um, 120um, 150um

  • Multiple-stream plate sorting

    • 4 streams on 96-well plate

    • 8 streams on 384-well plates

  • Equipped with nine lasers:

    • UV 349nm, 405nm, 445nm, 488nm, 532nm, 561nm, 594nm, 640nm, 785nm

  • 60 fluorescence channel detectors