The Neural Basis of Drosophila Courtship Song

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bioRxiv. 2023 Sep 01;. doi: 10.1101/2023.08.30.555537

The Neural Basis of Drosophila Courtship Song Dickson LabFlyLightFlyEMProject Technical Resources

Lillvis Joshua, Wang Kaiyu, Shiozaki Hiroshi, Xu Min, Stern David, Dickson Barry

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Abstract

Animal sounds are produced by patterned vibrations of specific organs, but the neural circuits that drive these vibrations are not well defined in any animal. Here we provide a functional and synaptic map of most of the neurons in the Drosophila male ventral nerve cord (the analog of the vertebrate spinal cord) that drive complex, patterned song during courtship. Male Drosophila vibrate their wings toward females during courtship to produce two distinct song modes – pulse and sine song – with characteristic features that signal species identity and male quality. We identified song-producing neural circuits by optogenetically activating and inhibiting identified cell types in the ventral nerve cord (VNC) and by tracing their patterns of synaptic connectivity in the male VNC connectome. The core song circuit consists of at least eight cell types organized into overlapping circuits, where all neurons are required for pulse song and a subset are required for sine song. The pulse and sine circuits each include a feed-forward pathway from brain descending neurons to wing motor neurons, with extensive reciprocal and feed-back connections. We also identify specific neurons that shape the individual features of each song mode. These results reveal commonalities amongst diverse animals in the neural mechanisms that generate diverse motor patterns from a single set of muscles.

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bioRxiv PrePrint https://doi.org/10.1101/2023.08.30.555537

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