iATPSnFR2

The iATPSnFR2 is a next-generation ATP sensor that offers significant improvements over its predecessor, iATPSnFR1, for real-time tracking of ATP levels within subcellular compartments. By optimizing the linkers between its domains, iATPSnFR2 achieves a 5-6 fold enhancement in its dynamic range. This sensor utilizes a modified GFP inserted within the ATP-binding helices of a bacterial ATPase subunit, ensuring high specificity and sensitivity to ATP while effectively discriminating against other analytes. With three affinity variants and options for ratiometric readout, iATPSnFR2 allows precise measurement of ATP dynamics across various cellular regions, making it a powerful tool for probing cellular metabolism, particularly in nerve terminals and mitochondria.

ATP is essential for cellular processes, and understanding its distribution and dynamics is crucial for studying cellular metabolism. Traditional biochemical methods have limitations in capturing these dynamics within living cells. Fluorescent biosensors like iATPSnFR2 bridge this gap by providing high temporal and spatial resolution of ATP levels. The iATPSnFR2 sensor builds on the foundation of earlier ATP sensors by enhancing the dynamic range and providing better suitability for real-time imaging in live cells. This improvement opens up new possibilities for studying metabolic processes under different physiological and pathological conditions.

Overall, iATPSnFR2 represents a significant advancement in the field of metabolic research. Its high dynamic range, specificity, and versatility in targeting subcellular locations make it an invaluable tool for researchers. By enabling detailed measurements of ATP variations and dynamics within cells, iATPSnFR2 can provide deeper insights into cellular energy regulation and metabolic responses, offering potential breakthroughs in understanding diseases and developing new treatments.

Benefits and Applications of iATPSnFR2:

• High Dynamic Range: Offers a 5-6 fold improvement in dynamic range over previous sensors.
• Specific and Sensitive: Provides excellent discrimination against other analytes and comes in three affinity variants.
• Versatile Targeting: Can be directed to specific subcellular locations, allowing detailed metabolic studies.
• Ratiometric Readout: Facilitates quantitative comparisons of ATP levels across different cellular regions and conditions.

Opportunity: iATPSnFR2 is available for commercial license (internal research and commercial sale) and for academic use via Addgene.

Intellectual Property: Pending patent  PCT/US2024/026558.