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Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics

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bioRxiv. 2025 Jul;. doi: 10.1101/2025.02.04.636286
Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics Harris Lab
Lakunina A, Socha KZ, Ladd A, Bowen AJ, Chen S, Colonell J, Doshi A, Karsh B, Krumin M, Kulik P, Li A, Neutens P, O’Callaghan J, Olsen M, Putzeys J, Tilmans HA, Ye Z, Welkenhuysen M, Häusser M, Koch C, Ting JT, Consortium Neuropixels, Dutta B, Harris TD, Steinmetz NA, Svoboda K, Siegle JH, Carandini M
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Abstract

High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations, and is especially powerful when combined with optogenetics for manipulation of specific cell types with high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic and photonic circuits. These devices pack 960 electrical recording sites and two sets of 14 light emitters onto a 1 cm shank, allowing spatially addressable optogenetic stimulation with blue and red light. In mouse cortex, Neuropixels Opto probes delivered high-quality recordings together with spatially addressable optogenetics, differentially activating or silencing neurons at distinct cortical depths. In mouse striatum and other deep structures, Neuropixels Opto probes delivered efficient optotagging, facilitating the identification of two cell types in parallel. Neuropixels Opto probes represent an unprecedented tool for recording, identifying, and manipulating neuronal populations.

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BioRvix Preprint: https://www.biorxiv.org/content/early/2025/02/06/2025.02.04.636286

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Janelia Authors

Susu Chen
Jennifer Colonell
Bill Karsh
Tim Harris
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