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Atalanta plasmid technology
New DNA plasmids for modification of genomes by homology-directed repair
Overview:
Next-gen DNA plasmids (pJAT series) for CRISPR-Cas9 genome modification using homology-directed repair (HDR)—originally developed for Drosophila, adaptable to other organisms.
Key Benefits:
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High HDR Efficiency: ~25% efficiency in injected eggs—significantly higher than traditional methods.
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One-Step Assembly: Combines dsDNA synthesis and Gateway cloning for rapid, simplified plasmid construction.
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All-in-One Design: Includes long homology arms, high-expression sgRNA, and large payload capacity.
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Modular & Customizable: Offers ready-to-use plasmids for common edits + empty vectors for tailored experiments.
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Broad Use: Effective in model and non-model species; enables complex genome edits like chromosomal inversions.
Use Cases:
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Targeted gene knock-ins
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Insertional mutagenesis
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Chromosomal rearrangements
Access & IP:
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Available via Addgene for academics -- see the link at the upper right
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Licensing available for commercial use
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Patent pending (US 18/736,729)
