Atalanta plasmid technology

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Atalanta plasmid technology

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New DNA plasmids for modification of genomes by homology-directed repair

Overview:
Next-gen DNA plasmids (pJAT series) for CRISPR-Cas9 genome modification using homology-directed repair (HDR)—originally developed for Drosophila, adaptable to other organisms.

Key Benefits:

High HDR Efficiency: ~25% efficiency in injected eggs—significantly higher than traditional methods.
One-Step Assembly: Combines dsDNA synthesis and Gateway cloning for rapid, simplified plasmid construction.
All-in-One Design: Includes long homology arms, high-expression sgRNA, and large payload capacity.
Modular & Customizable: Offers ready-to-use plasmids for common edits + empty vectors for tailored experiments.
Broad Use: Effective in model and non-model species; enables complex genome edits like chromosomal inversions.