Bright and Targetable Red Ca²⁺ Indicators "JF-BAPTA"

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Bright and Targetable Red Ca²⁺ Indicators "JF-BAPTA"

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Bright and Targetable Red Ca²⁺ Indicators with JF-BAPTA Technology

Overview

JF-BAPTA indicators merge the fluorescence power of Janelia Fluor dyes with the Ca²⁺ sensitivity of BAPTA and the genetic targeting precision of HaloTag. This next-generation sensor platform delivers high-performance calcium imaging in cytoplasmic and subcellular environments.

Key Features

High ΔF/F₀ and Quantum Yield: Up to 15× signal change and quantum yields as high as 0.75.
HaloTag Targeting: Enables selective labeling and precise subcellular imaging.
Spectral Flexibility: JF549 and JF646 derivatives offer compatibility with GFP, optogenetics, and deep-tissue imaging setups.
Far-Red Imaging of the Primary Cilium: First small-molecule red calcium dye to successfully label and detect dynamic calcium fluxes in the primary cilium.

Spotlight Indicators

JF549-BAPTA: Bright red indicator for general Ca²⁺ imaging
HaloTag-JF549-BAPTA: Targetable red probe for high signal with subcellular control
HaloTag-JF646-BAPTA: Far-red, fluorogenic probe ideal for primary cilia and deep tissue imaging