jGCaMP8 Calcium indicators

janelia7_blocks-janelia7_node_tool_type_list | block

Reagents

node_title | node_title

jGCaMP8 Calcium indicators

node:body | entity_field

New genetically encoded indicators for measuring fast Calcium transients in vitro and in vivo

The Looger Lab and the GENIE Project Team at HHMI Janelia have developed a new suite of jGCaMP8 Calcium indicators, built upon the successful GCaMP6 and jGCaMP7 scaffolds. The new jGCaMP8 sensors have fast kinetics and high sensitivity, suitable for in vivo imaging. Responses of the new sensors to field stimulation in cultured neurons are shown on the right. The sensors are:

jGCaMP8f (fast): 4x faster rise time, 2.5x faster decay time than jGCaMP7f
jGCaMP8m (medium): almost 4x faster rise time and 3.5x more sensitive than jGCaMP7f
jGCaMP8s (sensitive): 2x more sensitive than jGCaMP7s, >2x faster than jGCaMP7f (at 1 AP)
jGCaMP8.455, .543, .707, 712: fast and exhibit various promising characteristics for in vivo imaging. They are under examination currently.

Advantages

Allow imaging cell activity long-term and in vivo, unlike calcium dyes that are toxic to the cell.
Useful for genetically targeting expression

Applications

Neuroscience research using in vitro or in vivo models
Long-term functional imaging of neuronal activity and correlation with animal behavior
Screening for G-protein coupled receptor (GPCR) or ion channel agonists and antagonists
Cell, drosophila, zebrafish, mouse, and rat assays made possible through a multitude of variants and useful for imaging methods

Additional Information:

Figshare jGCaMP8 page
Janelia jGCaMP8 page

Opportunity:

Non-profit researchers can obtain jGCaMP8 variants at:

Plasmids and AAVs: Addgene.
Transgenic mice: JAX

The reagents are also available for commercial license (internal use or commercial sale) by contacting innovation@janelia.hhmi.org. Download our GCaMP Commercial Catalog!

node:field_image | entity_field