Main Menu (Mobile)- Block
- Overview
-
Support Teams
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing Software
- Scientific Computing Systems
- Viral Tools
- Vivarium
- Open Science
- You + Janelia
- About Us
Main Menu - Block
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing Software
- Scientific Computing Systems
- Viral Tools
- Vivarium
jGCaMP8 transgenic mice
Transgenic mice expressing jGCaMP8s and jGCaMP8m
The GENIE Project Team at HHMI Janelia Research Campus have developed and characterized multiple transgenic mice expressing jGCaMP8s and jGCaMP8m (see https://doi.org/10.25378/janelia.24565837) and deposited the lines at The Jackson Laboratory (JAX). jGCaMP8s and jGCaMP8m are genetically encoded calcium indicators with fast kinetics (~5 fold shorter rise time) than jGCaMP7f and high sensitivity (2X higher 1AP SNR) than jGCaMP7s (see https://doi.org/10.1038/s41586-023-05828-9 for details). The white paper/development report provides updates on developments in each transgenic mouse line, relevent data, and additional detail on how to obtain them.
The novel mouse lines include:
1) Tetracycline‐controlled transactivator protein (tTA)-dependent expression
| JAX # | Line | Camk2a-tTA |
|---|---|---|
| 037717 | TetO‐jGCaMP8s | Histology |
2) Cre recombinase-dependent expression
| JAX # | Line |
Slc17a7-Cre (Vglut1) |
Slc32a1-cre (Vgat) |
Slc17a7-cre/ Slc32a1-crea |
Snap25-cre |
|---|---|---|---|---|---|
| 037718 | TIGRE2‐jGCaMP8m‐IRES‐tTA2‐WPRE | N/A | N/A | N/A | |
| 037719 | TIGRE2‐jGCaMP8s‐IRES‐tTA2 | Histology | Histology | N/A | N/A |
| 037952 | TIGRE2-jGCaMP8s-IRES-tTA2-WPRE | Histology | Histology | N/A | N/A |
| 039267 | TIGRE2‐RiboL1-jGCaMP8s‐IRES‐tTA2 | Histologyb | Histology | Histology | Histologyc |
aDual cre drivers
bSome slices were only partially acquired.
cFor histology only, this cross displayed aberrant neural activity.
3) Thy1-jGCaMP8s
- Performance did not surpass GP5.17 (Thy1-GCaMP6f). GP5.17 is still recommended. (Histology for reference)
