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GENIE Reagents

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jGCaMP7 calcium indicators

Plasmids available from Addgene

Name	Description	Addgene reagent	Reference
pCMV-jGCaMP7s	improved SNR, slow kinetics	Plasmid	Dana et al., 2019
pCMV-jGCaMP7f	improved SNR, fast kinetics	Plasmid	Dana et al., 2019
pCMV-jGCaMP7b	improved SNR, higher baseline fluorescence	Plasmid	Dana et al., 2019
pCMV-jGCaMP7c	very low baseline fluorescence	Plasmid	Dana et al., 2019
pAAV-syn-jGCaMP7s-WPRE	improved SNR, slow kinetics	Plasmid AAV1 AAV9 AAVrg	Dana et al., 2019
pAAV-syn-jGCaMP7f-WPRE	improved SNR, fast kinetics	Plasmid AAV1 AAV8 AAV9 AAVrg	Dana et al., 2019
pAAV-syn-jGCaMP7b-WPRE	improved SNR, higher baseline fluorescence	Plasmid AAV1 AAV8	Dana et al., 2019
pAAV-syn-jGCaMP7c-WPRE	very low baseline fluorescence	Plasmid AAV1	Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7s-WPRE	improved SNR, slow kinetics, Cre-dependent expression	Plasmid AAV1 AAV9 PHPeB AAVrg	Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7f-WPRE	improved SNR, fast kinetics, Cre-dependent expression	Plasmid AAV1 AAVrg	Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7b-WPRE	improved SNR, higher baseline fluorescence, Cre-dependent expression	Plasmid AAV1 AAVrg	Dana et al., 2019
pAAV-syn-FLEX-jGCaMP7c-WPRE	very low baseline fluorescence, Cre-dependent expression	Plasmid AAV1	Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7s-WPRE	improved SNR, slow kinetics, Cre-dependent expression	Plasmid AAVrg	Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7f-WPRE	improved SNR, fast kinetics, Cre-dependent expression	Plasmid AAV1 AAVrg	Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7b-WPRE	improved SNR, higher baseline fluorescence, Cre-dependent expression	Plasmid	Dana et al., 2019
pAAV-CAG-FLEX-jGCaMP7c-WPRE	very low baseline fluorescence, Cre-dependent expression	Plasmid	Dana et al., 2019

Flies available from Bloomington Stock Center

Line name	Insertion Site	Bloomington Stock Number	Reference	Chr
P{20XUAS-IVS-Syn21-op1-jGCaMP7s-p10}	su(Hw)attP5	80905	Dana et al., 2019	2
P{20XUAS-IVS-Syn21-op1-jGCaMP7f-p10}	su(Hw)attP5	80906	Dana et al., 2019	2
P{20XUAS-IVS-Syn21-op1-jGCaMP7b-p10}	su(Hw)attP5	80907	Dana et al., 2019	2
P{20XUAS-IVS-Syn21-op1-jGCaMP7c-p10}	su(Hw)attP5	80908	Dana et al., 2019	2
P{20XUAS-IVS-Syn21-op1-jGCaMP7s-p10}	VK00005	79032	Dana et al., 2019	3
P{20XUAS-IVS-Syn21-op1-jGCaMP7f-p10}	VK00005	79031	Dana et al., 2019	3
P{20XUAS-IVS-Syn21-op1-jGCaMP7b-p10}	VK00005	79029	Dana et al., 2019	3
P{20XUAS-IVS-Syn21-op1-jGCaMP7c-p10}	VK00005	79030	Dana et al., 2019	3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7s-p10}	su(Hw)attP8	80909	Dana et al., 2019	X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7f-p10}	su(Hw)attP8	80910	Dana et al., 2019	X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7b-p10}	su(Hw)attP8	80911	Dana et al., 2019	X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7c-p10}	su(Hw)attP8	80912	Dana et al., 2019	X
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7s-p10}	VK00005	80913	Dana et al., 2019	3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7f-p10}	VK00005	80914	Dana et al., 2019	3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7b-p10}	VK00005	80915	Dana et al., 2019	3
P{13XLexAop2-IVS-Syn21-op1-jGCaMP7c-p10}	VK00005	80916	Dana et al., 2019	3

jRCaMP1 calcium indicators

Plasmids available from Addgene.org:

Name	Description	Addgene Plasmid ID	Reference
CMV-NES-jRCaMP1a	red, improved SNR, slower kinetics, bright	61562	Dana et al., 2016
CMV-NES-jRCaMP1b	red, improved SNR, improved dynamic range, bright	63136	Dana et al., 2016

Viruses available from Addgene

Name	Addgene virus	Reference
AAV1.Syn.NES.jRCaMP1a.WPRE.SV40	AAV1 AAV9	Dana et al., 2016
AAV1.CAG.Flex.NES.jRCaMP1a.WPRE.SV40	AAV1	Dana et al., 2016
AAV1.Syn.Flex.NES.jRCaMP1a.WPRE.SV40	AAV1 AAVrg	Dana et al., 2016
AAV1.Syn.NES.jRCaMP1b.WPRE.SV40	AAV1 AAV9	Dana et al., 2016
AAV1.CAG.Flex.NES.jRCaMP1b.WPRE.SV40	AAV1	Dana et al., 2016
AAV1.Syn.Flex.NES.jRCaMP1b.WPRE.SV40	AAV1	Dana et al., 2016

Flies available from Bloomington Stock Center:

Line name	Insertion Site	Bloomington Stock Number	Reference	Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1a-p10}	VK00005	63792	Dana et al., 2016	3
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1b-p10}	VK00005	63793	Dana et al., 2016	3

jRGECO1 calcium indicators

Plasmids available from Addgene.org:

Name	Description	Addgene Plasmid ID	Reference
CMV-NES-jRGECO1a	red, improved SNR	61563	Dana et al., 2016

Viruses available from University of Pennsylvania Viral Vector Core:

Name	Penn Catalog Number	Reference
AAV1.Syn.NES.jRGECO1a.WPRE.SV40	AV-1-PV3849	Dana et al., 2016
AAV1.CAG.Flex.NES.jRGECO1a.WPRE.SV40	not yet available	Dana et al., 2016
AAV1.Syn.Flex.NES.jRGECO1a.WPRE.SV40	AV-1-PV3848	Dana et al., 2016
AAV1.Syn.NES.jRGECO1b.WPRE.SV40	AV-1-PV3846	Dana et al., 2016
AAV1.CAG.Flex.NES.jRGECO1b.WPRE.SV40	not yet available	Dana et al., 2016
AAV1.Syn.Flex.NES.jRGECO1b.WPRE.SV40	not yet available	Dana et al., 2016

Mice available from The Jackson Laboratory:

Name	Description	Jax Stock Number	Reference
Thy1-jRGECO1a-WPRE (founder line GP8.20)	red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern	030525	Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.31)	red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern	030526	Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.58)	red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern	030527	Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.62)	red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern	030528	Dana et al., manuscript in preparation

Flies available from Bloomington Stock Center:

Line name	Insertion Site	Bloomington Stock Number	Reference	Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1a-p10}	VK00005	63794	Dana et al., 2016	3
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1b-p10}	VK00005	63795	Dana et al., 2016	3

GCaMP6 calcium indicators

Plasmids available from Addgene.org:

Name	Description	Addgene Plasmid ID	Reference
CMV-GCaMP6s	improved SNR, slower kinetics	40753	Chen et al., 2013
CMV-GCaMP6m	improved SNR, intermediate kinetics	40754	Chen et al., 2013
CMV-GCaMP6f	improved SNR, faster kinetics	40755	Chen et al., 2013

Viruses available from University of Pennsylvania Viral Vector Core:

Name	Penn Catalog Number	Penn Map and Sequence Number	Reference
AAV1.Syn.GCaMP6f.WPRE.SV40	AV-1-PV2822	Penn Vector P2822	Chen et al., 2013
AAV1.Syn.GCaMP6s.WPRE.SV40	AV-1-PV2824	Penn Vector P2824	Chen et al., 2013
AAV1.Syn.GCaMP6m.WPRE.SV40	AV-1-PV2823	Penn Vector P2823	Chen et al., 2013
AAV5.Syn.GCaMP6m.WPRE.SV40	AV-5-PV2823	Penn Vector P2823	Chen et al., 2013
AAV5.Syn.GCaMP6f.WPRE.SV40	AV-5-PV2822	Penn Vector P2822	Chen et al., 2013
AAV5.Syn.GCaMP6s.WPRE.SV40	AV-5-PV2824	Penn Vector P2824	Chen et al., 2013
AAV9.Syn.GCaMP6f.WPRE.SV40	AV-9-PV2822	Penn Vector P2822	Chen et al., 2013
AAV9.Syn.GCaMP6m.WPRE.SV40	AV-9-PV2823	Penn Vector P2823	Chen et al., 2013
AAV9.Syn.GCaMP6s.WPRE.SV40	AV-9-PV2824	Penn Vector P2824	Chen et al., 2013
AAV1.CAG.GCaMP6s.WPRE.SV40	AV-1-PV2833	Penn Vector P2833	Chen et al., 2013
AAV5.CAG.GCaMP6s.WPRE.SV40	AV-5-PV2833	Penn Vector P2833	Chen et al., 2013
AAV9.CAG.GCaMP6s.WPRE.SV40	AV-9-PV2833	Penn Vector P2833	Chen et al., 2013
AAV1.CAG.Flex.GCaMP6f.WPRE.SV40	AV-1-PV2816	Penn Vector 2816	Chen et al., 2013
AAV1.CAG.Flex.GCaMP6m.WPRE.SV40	AV-1-PV2817	Penn Vector 2817	Chen et al., 2013
AAV1.CAG.Flex.GCaMP6s.WPRE.SV40	AV-1-PV2818	Penn Vector 2818	Chen et al., 2013
AAV1.Syn.Flex.GCaMP6f.WPRE.SV40	AV-1-PV2819	Penn Vector 2819	Chen et al., 2013
AAV1.Syn.Flex.GCaMP6m.WPRE.SV40	AV-1-PV2820	Penn Vector 2820	Chen et al., 2013
AAV1.Syn.Flex.GCaMP6s.WPRE.SV40	AV-1-PV2821	Penn Vector 2821	Chen et al., 2013
AAV5.CAG.Flex.GCaMP6f.WPRE.SV40	AV-5-PV2816	Penn Vector 2816	Chen et al., 2013
AAV5.CAG.Flex.GCaMP6m.WPRE.SV40	AV-5-PV2817	Penn Vector 2817	Chen et al., 2013
AAV5.CAG.Flex.GCaMP6s.WPRE.SV40	AV-5-PV2818	Penn Vector 2818	Chen et al., 2013
AAV5.Syn.Flex.GCaMP6f.WPRE.SV40	AV-5-PV2819	Penn Vector 2819	Chen et al., 2013
AAV5.Syn.Flex.GCaMP6m.WPRE.SV40	AV-5-PV2820	Penn Vector 2820	Chen et al., 2013
AAV5.Syn.Flex.GCaMP6s.WPRE.SV40	AV-5-PV2821	Penn Vector 2821	Chen et al., 2013
AAV9.CAG.Flex.GCaMP6f.WPRE.SV40	AV-9-PV2816	Penn Vector 2816	Chen et al., 2013
AAV9.CAG.Flex.GCaMP6m.WPRE.SV40	AV-9-PV2817	Penn Vector 2817	Chen et al., 2013
AAV9.CAG.Flex.GCaMP6s.WPRE.SV40	AV-9-PV2818	Penn Vector 2818	Chen et al., 2013
AAV9.Syn.Flex.GCaMP6f.WPRE.SV40	AV-9-PV2819	Penn Vector 2819	Chen et al., 2013
AAV9.Syn.Flex.GCaMP6m.WPRE.SV40	AV-9-PV2820	Penn Vector 2820	Chen et al., 2013
AAV9.Syn.Flex.GCaMP6s.WPRE.SV40	AV-9-PV2821	Penn Vector 2821	Chen et al., 2013

Mice available from The Jackson Laboratory:

Name	Description	Jax Stock Number	Reference
Thy1-GCaMP6s-WPRE (founder line GP4.3)	improved SNR, slower kinetics, variable neuronal expression pattern	024275	Dana et al., 2015
Thy1-GCaMP6s-WPRE (founder line GP4.6)	improved SNR, slower kinetics, variable neuronal expression pattern	028278	Dana et al., 2015
Thy1-GCaMP6s-WPRE (founder line GP4.12)	improved SNR, slower kinetics, variable neuronal expression pattern	025776	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.1)	improved SNR, faster kinetics, variable neuronal expression pattern	028279	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.3)	improved SNR, faster kinetics, variable neuronal expression pattern	028280	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.5)	improved SNR, faster kinetics, variable neuronal expression pattern	024276	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.11)	improved SNR, faster kinetics, variable neuronal expression pattern	024339	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.12)	improved SNR, faster kinetics, variable neuronal expression pattern	028281	Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.17)	improved SNR, faster kinetics, variable neuronal expression pattern	025393	Dana et al., 2015

Flies available from Bloomington Stock Center:

Line name	Insertion Site	Bloomington Stock Number	Reference	Chr
P{20XUAS-IVS-GCaMP6s}	attP40	42476	Chen et al., 2013	2
P{20XUAS-IVS-GCaMP6m}	attP40	42748	Chen et al., 2013	2
P{20XUAS-IVS-GCaMP6f}	attP40	42747	Chen et al., 2013	2
PBac{20XUAS-IVS-GCaMP6s}	VK00005	42749	Chen et al., 2013	3
PBac{20XUAS-IVS-GCaMP6m}	VK00005	42750	Chen et al., 2013	3
P{13XLexAop2-IVS-GCaMP6s-SV40}	su(Hw)attP1	44273	Chen et al., 2013	3
P{13XLexAop2-IVS-GCaMP6m-SV40}	su(Hw)attP1	44588	Chen et al., 2013	3
P{13XLexAop2-IVS-GCaMP6s-SV40}	su(Hw)attP5	44589	Chen et al., 2013	2
P{13XLexAop2-IVS-GCaMP6s-p10}	su(Hw)attP1	44274	Chen et al., 2013	3
P{13XLexAop2-IVS-GCaMP6m-p10}	su(Hw)attP1	44275	Chen et al., 2013	3
P{13XLexAop2-IVS-GCaMP6s-p10}	su(Hw)attP5	44590	Chen et al., 2013	2
P{13XLexAop2-IVS-GCaMP6f-p10}	su(Hw)attP5	44277	Chen et al., 2013	2
P{13XLexAop2-IVS-GCaMP6m-p10}	VK00005	44276	Chen et al., 2013	3

C. elegans distributed upon request

Name
mec-4::nls-RSET-GCaMP6s:SL2:nls-TagRFP::unc-54utr

GCaMP5 calcium indicators

Plasmids available from Addgene.org:

Name	Description	Addgene Plasmid ID	Reference
CMV-GCaMP5G	Green calcium indicator	31788	Akerboom et al., 2012

Viruses available from University of Pennsylvania Viral Vector Core:

Name	Penn Catalog Number	Penn Map and Sequence Number	Reference
AAV1.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-1-PV2478	Penn Vector P2478	Akerboom et al., 2013
AAV5.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-5-PV2478	Penn Vector P2478	Akerboom et al., 2013
AAV9.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-9-PV2478	Penn Vector P2478	Akerboom et al., 2013
AAV9.CAG.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-9-PV2643	Penn Vector P2643	Akerboom et al., 2013
AAV1.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-1-PV2541	Penn Vector P2541	Akerboom et al., 2013
AAV9.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-9-PV2541	Penn Vector P2541	Akerboom et al., 2013
AAV1.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-1-PV2540	Penn Vector 2540	Akerboom et al., 2013
AAV5.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-5-PV2540	Penn Vector P2540	Akerboom et al., 2013
AAV9.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40	AV-9-PV2540	Penn Vector P2540	Akerboom et al., 2013

Flies available from Bloomington Stock Center:

Name	Insertion Site	Bloomington Stock Number	Reference	Chr
P{20XUAS-IVS-GCaMP5G}	attP40	42037	Akerboom et al., 2012	2
P{20XUAS-IVS-GCaMP5G}	VK00005	42038	Akerboom et al., 2012	3

GCaMP3 calcium indicators

Plasmids available from Addgene.org:

Name	Description	Addgene Plasmid ID	Reference
CMV-GCaMP3	Green calcium indicator	22692	Tian et al., 2009

Viruses available from University of Pennsylvania Viral Vector Core:

Name	Penn Catalog Number	Penn Map and Sequence Number	Reference
AAV1.hSynap.GCaMP3.WPRE.SV40	AV-1-PV1627	Penn Vector P1627	Tian et al., 2013
AAV5.hSynap.GCaMP3.WPRE.SV40	AV-5-PV1627	Penn Vector P1627	Tian et al., 2013
AAV9.hSynap.GCaMP3.WPRE.SV40	AV-9-PV1627	Penn Vector P1627	Tian et al., 2013
AAV1.hSynap.Flex.GCaMP3.WPRE.SV40	AV-1-PV1921	Penn Vector P1921	Tian et al., 2013
AAV5.hSynap.Flex.GCaMP3.WPRE.SV40	AV-5-PV1921	Penn Vector P1921	Tian et al., 2013
AAV9.hSynap.Flex.GCaMP3.WPRE.SV40	AV-9-PV1921	Penn Vector P1921	Tian et al., 2013

Mice available from The Jackson Laboratory:

Name	Jax Stock Number	Reference
Rosa26-CAG-lox-stop-lox-GCaMP3-WPRE	014538	Zariwala et al., 2010

Flies available from Bloomington Stock Center:

Name	Insertion Site	Bloomington Stock Number	Reference	Chr
P{UAS-GCaMP3.T}	attP40	32116	Tian et al., 2009	2
P{20XUAS-GCaMP3}	su(Hw)attP8	32234	Tian et al., 2009	1
P{20XUAS-GCaMP3}	attP18	32235	Tian et al., 2009	1
P{20XUAS-GCaMP3}	attP2	32236	Tian et al., 2009	3
PBac{20XUAS-GCaMP3}	VK00005	32237	Tian et al., 2009	3

Data and reagent release policy

We distribute reagents within nine months after initial discovery, often long before publication.

The data characterizing reagents is published and/or made available on this website. DNA constructs will be deposited at Addgene; viruses at the University of Pennsylvania Vector Core; mice at The Jackson Laboratory; Drosophila at the Bloomington Drosophila Stock Center. C. elegans lines will be distributed upon request.

Reagents can also be made available to collaborators who agree to testing sensor variants using rigorous procedures with jointly agreed upon standards of merit.

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GENIE Reagents

jGCaMP7 calcium indicators

Plasmids available from Addgene

Flies available from Bloomington Stock Center

jRCaMP1 calcium indicators

jRGECO1 calcium indicators

GCaMP6 calcium indicators

GCaMP5 calcium indicators

GCaMP3 calcium indicators

Data and reagent release policy

We distribute reagents within nine months after initial discovery, often long before publication.

GENIE reagents

jGCaMP7 calcium indicators

jRCaMP1 calcium indicators

jRGECO1 calcium indicators

GCaMP6 calcium indicators

GCaMP5 calcium indicators

GCaMP3 calcium indicators

Data and reagent release policy

Policy