A genetically encoded fluorescent sensor for in vivo imaging of GABA.

janelia7_blocks-janelia7_biblio_header | block

Nature Methods. 2019 Jul 15;16(8):763-770. doi: 10.1038/s41592-019-0471-2

A genetically encoded fluorescent sensor for in vivo imaging of GABA. Podgorski LabAhrens LabLooger Lab

Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Current techniques for monitoring GABA (γ-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.

PMID: 31308547 [PubMed - indexed for MEDLINE]

node:body | entity_field

bioRxiv PrePrint https://doi.org/10.1101/322578 (May 14, 2018)

janelia7_blocks-janelia7_biblio_authors | block

janelia7_blocks-janelia7_biblio_tools | block

Main Menu (Mobile)- Block

Main Menu - Block

A genetically encoded fluorescent sensor for in vivo imaging of GABA.

Abstract

PMID: 31308547 [PubMed - indexed for MEDLINE]

Janelia Authors