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janelia7_blocks-janelia7_biblio_header | block
Nature Methods. Jan-12-2017;14(12):1167 - 1170. doi: 10.1038/nmeth.4465
Antibodies to biotin enable large-scale detection of biotinylation sites on proteins Pedram Lab
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Udeshi ND, Pedram K, Svinkina T, Fereshetian S, Myers SA, Aygun O, Krug K, Clauser K, Ryan D, Ast T, Mootha VK, Ting AY, Carr SA
Note: Research in this publication was not performed at Janelia.
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that anti-biotin antibodies enable unprecedented enrichment of biotinylated peptides from complex peptide mixtures. Live-cell proximity labeling using APEX peroxidase followed by anti-biotin enrichment and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-based enrichment of proteins.
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janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
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