Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

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Nature Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178

Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. Gonen Lab

de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T

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Abstract

Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

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Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

Abstract

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