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janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. Gonen Lab
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de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
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Abstract
Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
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Janelia Authors
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