Breaking the diffraction barrier: optical microscopy on a nanometric scale.

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Science. 1991 Mar 22;251(5000):1468-70. doi: 10.1126/science.251.5000.1468

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Breaking the diffraction barrier: optical microscopy on a nanometric scale. Betzig LabHarris Lab

Betzig E, Trautman JK, Harris TD, Weiner JS, Kostelak RL

Note: Research in this publication was not performed at Janelia.

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Abstract

In near-field scanning optical microscopy, a light source or detector with dimensions less than the wavelength (lambda) is placed in close proximity (lambda/50) to a sample to generate images with resolution better than the diffraction limit. A near-field probe has been developed that yields a resolution of approximately 12 nm ( approximately lambda/43) and signals approximately 10(4)- to 10(6)-fold larger than those reported previously. In addition, image contrast is demonstrated to be highly polarization dependent. With these probes, near-field microscopy appears poised to fulfill its promise by combining the power of optical characterization methods with nanometric spatial resolution.

PMID: 17779440 [PubMed - indexed for MEDLINE]

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Breaking the diffraction barrier: optical microscopy on a nanometric scale.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 17779440 [PubMed - indexed for MEDLINE]