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Microscopy and Microanalysis. 2025 Jul 25;31(1(Supp)):ozaf048.484. doi: 10.1093/mam/ozaf048.484
Cryo-electron Microscopy of Vitreous Sections (CEMOVIS) application for model organisms Cryo-Electron MicroscopyElectron Microscopy
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Abstract
Cryo-ultramicrotomy, developed by Bernhard in 1965 [1], has long been regarded as the pinnacle of achievement for electron microscopists. This technique allows biological samples to be sliced into ultrathin sections and examined in a cryo-electron microscope, revealing the most intricate subcellular structures without chemical fixation or staining. The advent of vitrification [2,3] and high-pressure freezing (HPF) technology [4,5] provided reliable methods for preserving cellular structures, and the introduction of diamond knife to cryo-ultramicrotomy [6] offering cryo-ultramicrotomists reassurance in consistency of the quality [7].
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Janelia Authors
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