Main Menu (Mobile)- Block
MENU
- Overview
-
Support Teams
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- High Performance Computing
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing
- Viral Tools
- Vivarium
- Open Science
- You + Janelia
- About Us
Labs:
Project Teams:
Main Menu - Block
Labs:
Project Teams:
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- High Performance Computing
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing
- Viral Tools
- Vivarium
janelia7_blocks-janelia7_biblio_header | block
Microscopy and Microanalysis. 2025 Jul 01;31(1(Supp)):ozaf048.484. doi: 10.1093/mam/ozaf048.484
Cryo-electron Microscopy of Vitreous Sections (CEMOVIS) Application for Model Organisms Cryo-Electron MicroscopyElectron Microscopy
Uryu K, Zhao X, Shiozaki M, Yu Z
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
Cryo-ultramicrotomy, developed by Bernhard in 1965 [1], has long been regarded as the pinnacle of achievement for electron microscopists. This technique allows biological samples to be sliced into ultrathin sections and examined in a cryo-electron microscope, revealing the most intricate subcellular structures without chemical fixation or staining. The advent of vitrification [2,3] and high-pressure freezing (HPF) technology [4,5] provided reliable methods for preserving cellular structures, and the introduction of diamond knife to cryo-ultramicrotomy [6] offering cryo-ultramicrotomists reassurance in consistency of the quality [7].
janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
janelia7_blocks-janelia7_biblio_tools | block