Elastic volume reconstruction from series of ultra-thin microscopy sections.

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Nature Methods. 2012 Jul;9(7):717-20. doi: 10.1038/nmeth.2072

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Elastic volume reconstruction from series of ultra-thin microscopy sections. Cardona LabSaalfeld LabFetter Lab

Saalfeld S, Fetter RD, Cardona A, Tomancak P

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Abstract

Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.

PMID: 22688414 [PubMed - indexed for MEDLINE]

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Elastic volume reconstruction from series of ultra-thin microscopy sections.

Abstract

PMID: 22688414 [PubMed - indexed for MEDLINE]

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