Main Menu (Mobile)- Block

Main Menu - Block

Even illumination in total internal reflection fluorescence microscopy using laser light.

Our Research / Publications
janelia7_blocks-janelia7_biblio_header | block
Microscopy Research and Technique. 2008 Jan;71(1):45-50. doi: 10.1002/jemt.20527
View Article on
Even illumination in total internal reflection fluorescence microscopy using laser light.
Fiolka R, Belyaev Y, Ewers H, Stemmer A
Note: Research in this publication was not performed at Janelia.
janelia7_blocks-janelia7_biblio_abstract | block

Abstract

In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi-illumination modes. In wide-field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform illumination of the specimen, compromising image analysis. We report the design and construction of an objective-launch total internal reflection fluorescence microscopy system with excellent evenness of specimen illumination achieved by azimuthal rotation of the incoming illuminating laser beam. The system allows quick and precise changes of the incidence angle of the laser beam and thus can also be used in an epifluorescence mode.

PMID: 17886344 [PubMed - indexed for MEDLINE]

node:body | entity_field
janelia7_blocks-janelia7_biblio_tools | block