Fast and accurate spike sorting of high-channel count probes with KiloSort.

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Neural Information Processing Systems (NIPS 2016). 2016 Dec 05

Fast and accurate spike sorting of high-channel count probes with KiloSort. Pachitariu Lab

Pachitariu M, Steinmetz NA, Kadir SN, Carandini M, Harris KD

Note: Research in this publication was not performed at Janelia.

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Abstract

New silicon technology is enabling large-scale electrophysiological recordings in vivo from hundreds to thousands of channels. Interpreting these recordings requires scalable and accurate automated methods for spike sorting, which should minimize the time required for manual curation of the results. Here we introduce KiloSort, a new integrated spike sorting framework that uses template matching both during spike detection and during spike clustering. KiloSort models the electrical voltage as a sum of template waveforms triggered on the spike times, which allows overlapping spikes to be identified and resolved. Unlike previous algorithms that compress the data with PCA, KiloSort operates on the raw data which allows it to construct a more accurate model of the waveforms. Processing times are faster than in previous algorithms thanks to batch-based optimization on GPUs. We compare KiloSort to an established algorithm and show favorable performance, at much reduced processing times. A novel post-clustering merging step based on the continuity of the templates further reduced substantially the number of manual operations required on this data, for the neurons with near-zero error rates, paving the way for fully automated spike sorting of multichannel electrode recordings.

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Fast and accurate spike sorting of high-channel count probes with KiloSort.

Note: Research in this publication was not performed at Janelia.

Abstract