Fluorogenic Labeling Strategies for Biological Imaging

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International Journal of Molecular Sciences. 2017 Jul;18:1473 – 11. doi: 10.3390/ijms18071473

Fluorogenic Labeling Strategies for Biological Imaging Tebo Lab

Li C, Tebo AG, Gautier A

Note: Research in this publication was not performed at Janelia.

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Abstract

The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy.

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Fluorogenic Labeling Strategies for Biological Imaging

Note: Research in this publication was not performed at Janelia.

Abstract

Janelia Authors