A full-length cDNA resource for the pea aphid, acyrthosiphon pisum.

Our Research / Publications

janelia7_blocks-janelia7_biblio_header | block

Insect Molecular Biology. 2010 Mar;19 Suppl 2:23-31. doi: 10.1111/j.1365-2583.2009.00946.x

View Article on

A full-length cDNA resource for the pea aphid, acyrthosiphon pisum. Stern Lab

Shigenobu S, Richards S, Cree AG, Morioka M, Fukatsu T, Kudo T, Miyagishima S, Gibbs RA, Stern DL, Nakabachi A

Note: Research in this publication was not performed at Janelia.

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5’ and 3’ ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.

PMID: 20482637 [PubMed - indexed for MEDLINE]

node:body | entity_field

Main Menu (Mobile)- Block

Main Menu - Block

A full-length cDNA resource for the pea aphid, acyrthosiphon pisum.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 20482637 [PubMed - indexed for MEDLINE]

Janelia Authors