High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.

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Cell Reports. 2021 Apr 06;35(1):108954. doi: 10.1016/j.celrep.2021.108954

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High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo. Svoboda Lab

Xie ME, Adam Y, Fan LZ, Böhm UL, Kinsella I, Zhou D, Rozsa M, Singh A, Svoboda K, Paninski L, Cohen AE

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Abstract

The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for this purpose, and recent developments have achieved a high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources of noise and signal extraction remains a challenge. We present an improved signal extraction pipeline, spike-guided penalized matrix decomposition-nonnegative matrix factorization (SGPMD-NMF), which resolves supra- and subthreshold voltages in vivo. The method incorporates biophysical and optical constraints. We validate the pipeline with simultaneous patch-clamp and optical recordings from mouse layer 1 in vivo and with simulated and composite datasets with realistic noise. We demonstrate applications to mouse hippocampus expressing paQuasAr3-s or SomArchon1, mouse cortex expressing SomArchon1 or Voltron, and zebrafish spines expressing zArchon1.

PMID: 33826882 [PubMed - indexed for MEDLINE]

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High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.

Abstract

PMID: 33826882 [PubMed - indexed for MEDLINE]

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