Large-scale brain-wide neural recording in nonhuman primates

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bioRxiv. 2023 Feb 04;. doi: 10.1101/2023.02.01.526664

Large-scale brain-wide neural recording in nonhuman primates Harris Lab

Trautmann Eric, Hesse Janis, Stine Gabriel, Xia Ruobing, Zhu Shude, O’Shea Daniel, Karsh Bill, Colonell Jennifer, Lanfranchi Frank, Vyas Saurabh, Zimnik Andrew, Steinmann Natalie, Wagenaar Daniel, Andrei Alexandru, Lopez Carolina, O’Callaghan John, Putzeys Jan, Raducanu Bogdan, Welkenhuysen Marleen, Churchland Mark, Moore Tirin, Shadlen Michael, Shenoy Krishna, Tsao Doris, Dutta Barundeb, Harris Timothy

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Abstract

High-density, integrated silicon electrodes have begun to transform systems neuroscience, by enabling large-scale neural population recordings with single cell resolution. Existing technologies, however, have provided limited functionality in nonhuman primate species such as macaques, which offer close models of human cognition and behavior. Here, we report the design, fabrication, and performance of Neuropixels 1.0-NHP, a high channel count linear electrode array designed to enable large-scale simultaneous recording in superficial and deep structures within the macaque or other large animal brain. These devices were fabricated in two versions: 4416 electrodes along a 45 mm shank, and 2496 along a 25 mm shank. For both versions, users can programmably select 384 channels, enabling simultaneous multi-area recording with a single probe. We demonstrate recording from over 3000 single neurons within a session, and simultaneous recordings from over 1000 neurons using multiple probes. This technology represents a significant increase in recording access and scalability relative to existing technologies, and enables new classes of experiments involving fine-grained electrophysiological characterization of brain areas, functional connectivity between cells, and simultaneous brain-wide recording at scale.

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bioRxiv PrePrint https://doi.org/10.1101/2023.02.01.526664

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Large-scale brain-wide neural recording in nonhuman primates

Abstract

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