Main Menu (Mobile)- Block

Main Menu - Block

larvalign: Aligning gene expression patterns from the larval brain of Drosophila melanogaster.

Our Research / Publications
janelia7_blocks-janelia7_biblio_header | block
Neuroinformatics. 2018 Jan 1;16(1):65-80. doi: 10.1007/s12021-017-9349-6
View Article on
larvalign: Aligning gene expression patterns from the larval brain of Drosophila melanogaster. Truman Lab
Muenzing SE, Strauch M, Truman JW, Bühler K, Thum AS, Merhof D
janelia7_blocks-janelia7_biblio_abstract | block

Abstract

The larval brain of the fruit fly Drosophila melanogaster is a small, tractable model system for neuroscience. Genes for fluorescent marker proteins can be expressed in defined, spatially restricted neuron populations. Here, we introduce the methods for 1) generating a standard template of the larval central nervous system (CNS), 2) spatial mapping of expression patterns from different larvae into a reference space defined by the standard template. We provide a manually annotated gold standard that serves for evaluation of the registration framework involved in template generation and mapping. A method for registration quality assessment enables the automatic detection of registration errors, and a semi-automatic registration method allows one to correct registrations, which is a prerequisite for a high-quality, curated database of expression patterns. All computational methods are available within the larvalign software package: https://github.com/larvalign/larvalign/releases/tag/v1.0.

PMID: 29127664 [PubMed - indexed for MEDLINE]

node:body | entity_field
janelia7_blocks-janelia7_biblio_authors | block

Janelia Authors

Jim Truman
janelia7_blocks-janelia7_biblio_tools | block