Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.

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Nature Biotechnology. 2016 Jul 4;34(9):987-92. doi: 10.1038/nbt.3625

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Tillberg LabTranscription Imaging

Tillberg P, Chen F, Piatkevich KD, Zhao Y, Yu CJ, English BP, Gao L, Martorell A, Suk H, Yoshida F, DeGennaro EM, Roossien DH, Gong G, Seneviratne U, Tannenbaum SR, Desimone R, Cai D, Boyden ES

Note: Research in this publication was not performed at Janelia.

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Abstract

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (∼70 nm) imaging of cells and mammalian tissues on conventional microscopes.

PMID: 27376584 [PubMed - indexed for MEDLINE]

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Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 27376584 [PubMed - indexed for MEDLINE]

Janelia Authors