Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR.

janelia7_blocks-janelia7_biblio_header | block

Nature Methods. 2018 Nov;15(11):9386-9. doi: 10.1038/s41592-018-0171-3

Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR. Looger LabPodgorski LabLavis LabTool Translation Team (T3)

Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Mueller JA, Schoch-McGovern S, Wang SS, Quiroz FJ, Rebola N, Bao H, Little JP, Tkachuk AN, Hantman AW, Chapman ER, Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.