Stochastic electrotransport selectively enhances the transport of highly electromobile molecules.

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Proceedings of the National Academy of Sciences of the United States of America. 2015 Nov 2;112(46):E6274-83. doi: 10.1073/pnas.1510133112

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Stochastic electrotransport selectively enhances the transport of highly electromobile molecules. Keller Lab

Kim S, Cho JH, Murray E, Bakh N, Choi H, Ohn K, Ruelas L, Hubbert A, McCue M, Vassallo SL, Keller PJ, Chung K

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Abstract

Nondestructive chemical processing of porous samples such as fixed biological tissues typically relies on molecular diffusion. Diffusion into a porous structure is a slow process that significantly delays completion of chemical processing. Here, we present a novel electrokinetic method termed stochastic electrotransport for rapid nondestructive processing of porous samples. This method uses a rotational electric field to selectively disperse highly electromobile molecules throughout a porous sample without displacing the low-electromobility molecules that constitute the sample. Using computational models, we show that stochastic electrotransport can rapidly disperse electromobile molecules in a porous medium. We apply this method to completely clear mouse organs within 1–3 days and to stain them with nuclear dyes, proteins, and antibodies within 1 day. Our results demonstrate the potential of stochastic electrotransport to process large and dense tissue samples that were previously infeasible in time when relying on diffusion.

PMID: 26578787 [PubMed - indexed for MEDLINE]

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Stochastic electrotransport selectively enhances the transport of highly electromobile molecules.

Abstract

PMID: 26578787 [PubMed - indexed for MEDLINE]

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