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janelia7_blocks-janelia7_biblio_header | block
Methods in Molecular Biology (Clifton, N.J.). 2017;1663:179-188. doi: 10.1007/978-1-4939-7265-4_15
Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Lavis LabTranscription ImagingTool Translation Team (T3)
Grimm JB, Brown TA, English BP, Lionnet T, Lavis LD
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
The development of genetically encoded self-labeling protein tags such as the HaloTag and SNAP-tag has expanded the utility of chemical dyes in microscopy. Intracellular labeling using these systems requires small, cell-permeable dyes with high brightness and photostability. We recently discovered a general method to improve the properties of classic fluorophores by replacing N,N-dimethylamino groups with four-membered azetidine rings to create the "Janelia Fluor" dyes. Here, we describe the synthesis of the HaloTag and SNAP-tag ligands of Janelia Fluor 549 and Janelia Fluor 646 as well as standard labeling protocols for use in ensemble and single-molecule cellular imaging.
PMID: 28924668 [PubMed - indexed for MEDLINE]
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janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
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