Using translational enhancers to increase transgene expression in Drosophila.

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Proceedings of the National Academy of Sciences of the United States of America. 2012 Apr 9;109(17):6626-31. doi: 10.1073/pnas.1204520109

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Using translational enhancers to increase transgene expression in Drosophila. Truman LabRubin Lab

Pfeiffer BD, Truman JW, Rubin GM

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Abstract

The ability to specify the expression levels of exogenous genes inserted in the genomes of transgenic animals is critical for the success of a wide variety of experimental manipulations. Protein production can be regulated at the level of transcription, mRNA transport, mRNA half-life, or translation efficiency. In this report, we show that several well-characterized sequence elements derived from plant and insect viruses are able to function in Drosophila to increase the apparent translational efficiency of mRNAs by as much as 20-fold. These increases render expression levels sufficient for genetic constructs previously requiring multiple copies to be effective in single copy, including constructs expressing the temperature-sensitive inactivator of neuronal function Shibire(ts1), and for the use of cytoplasmic GFP to image the fine processes of neurons.

PMID: 22493255 [PubMed - indexed for MEDLINE]

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Using translational enhancers to increase transgene expression in Drosophila.

Abstract

PMID: 22493255 [PubMed - indexed for MEDLINE]

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