Variegated gene expression caused by cell-specific long-range DNA interactions.

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Nature Cell Biology. 2011 Aug;13(8):944-51. doi: 10.1038/ncb2278

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Variegated gene expression caused by cell-specific long-range DNA interactions. Singer Lab

Noordermeer D, de Wit E, Klous P, van de Werken H, Simonis M, Lopez-Jones M, Eussen B, de Klein A, Singer RH, de Laat W

Note: Research in this publication was not performed at Janelia.

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Abstract

Mammalian genomes contain numerous regulatory DNA sites with unknown target genes. We used mice with an extra β-globin locus control region (LCR) to investigate how a regulator searches the genome for target genes. We find that the LCR samples a restricted nuclear subvolume, wherein it preferentially contacts genes controlled by shared transcription factors. No contacted gene is detectably upregulated except for endogenous β-globin genes located on another chromosome. This demonstrates genetically that mammalian trans activation is possible, but suggests that it will be rare. Trans activation occurs not pan-cellularly, but in 'jackpot' cells enriched for the interchromosomal interaction. Therefore, cell-specific long-range DNA contacts can cause variegated expression.

PMID: 21706023 [PubMed - indexed for MEDLINE]

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Variegated gene expression caused by cell-specific long-range DNA interactions.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 21706023 [PubMed - indexed for MEDLINE]

Janelia Authors