ZCCHC8 is required for the degradation of pervasive transcripts originating from multiple genomic regulatory features

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bioRxiv. 2021 Jul;. doi: 10.1101/2021.01.29.428898

ZCCHC8 is required for the degradation of pervasive transcripts originating from multiple genomic regulatory features Wang (Shaohe) Lab

Collins Joshua, Martin Daniel, Genomics , Core Computational, Wang Shaohe, Yamada Kenneth

Note: Research in this publication was not performed at Janelia.

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Abstract

The vast majority of mammalian genomes are transcribed as non-coding RNA in what is referred to as “pervasive transcription.” Recent studies have uncovered various families of non-coding RNA transcribed upstream of transcription start sites. In particular, highly unstable promoter upstream transcripts known as PROMPTs have been shown to be targeted for exosomal degradation by the nuclear exosome targeting complex (NEXT) consisting of the RNA helicase MTR4, the zinc-knuckle scaffold ZCCHC8, and the RNA binding protein RBM7. Here, we report that in addition to its known RNA substrates, ZCCHC8 is required for the targeted degradation of pervasive transcripts produced at CTCF binding sites, open chromatin regions, promoters, promoter flanking regions, and transcription factor binding sites. Additionally, we report that a significant number of RIKEN cDNAs and predicted genes display the hallmarks of PROMPTs and are also substrates for ZCCHC8 and/or NEXT complex regulation suggesting these are unlikely to be functional genes. Our results suggest that ZCCHC8 and/or the NEXT complex may play a larger role in the global regulation of pervasive transcription than previously reported.Competing Interest StatementThe authors have declared no competing interest.

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ZCCHC8 is required for the degradation of pervasive transcripts originating from multiple genomic regulatory features

Note: Research in this publication was not performed at Janelia.

Abstract

Janelia Authors