3D CLEM Publications

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04/13/21 | Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insectsER-to-Golgi protein delivery through an interwoven, tubular network extending from ER.

Aubrey V Weigel, Chi-Lun Chang, Gleb Shtengel, C Shan Xu, David P Hoffman, Melanie Freeman, Nirmala Iyer, Jesse Aaron, Satya Khuon, John Bogovic, Wei Qiu, Harald F Hess, Jennifer Lippincott-Schwartz.
Cell. 2021 April 13; ISSN 0092-8674, doi: 10.1016/j.cell.2021.03.035

01/16/20 | Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells. Click to free download [Full text] [Reprint] [Abstract]

David P. Hoffman, Gleb Shtengel, C. Shan Xu, Kirby R. Campbell, Melanie Freeman, Lei Wang, Daniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, John A. Bogovic, Daniel R. Stabley, Abbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J. Solecki, Eric Betzig, Harald F. Hess
Science. 2020, Jan 17; 367(6475):eaaz5357 doi: 10.1126/science.aaz5357 [Cover Page]

10/14/15 | Correlative super-resolution optical and electron microscopy roadmap.

Shtengel G, Hess HF

Journal of Physics D: Applied Physics. 2015 Oct 14;48:443001. doi: 10.1088/0022-3727/48/44/443001

03/01/15 | Fixation-resistant photoactivatable fluorescent proteins for CLEM.

Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL

Nature Methods. 2015 Mar;12(3):215-8. doi: 10.1038/nmeth.3225

01/26/14 | Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.

Sochacki KA, Shtengel G, Van Engelenburg SB, Hess HF, Taraska JW

Nature Methods. 2014 Jan 26;11(3):305-8. doi: 10.1038/nmeth.2816

01/16/14 | Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.

Van Engelenburg SB, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan SD, Freed EO, Hess HF, Lippincott-Schwartz J

Science. 2014 Jan 16;343(6171):653-6. doi: 10.1126/science.1247786

01/01/14 | Imaging cellular ultrastructure by PALM, iPALM, and correlative iPALM-EM.

Shtengel G, Wang Y, Zhang Z, Goh WIng, Hess HF, Kanchanawong P

Methods in Cell Biology. 2014;123:273-94. doi: 10.1016/B978-0-12-420138-5.00015-X

10/25/13 | Correlative photoactivated localization and scanning electron microscopy.

Kopek BG, Shtengel G, Grimm JB, Clayton DA, Hess HF