Chamber and seals for multi-view microscopy

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Laboratory Tool

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Chamber and seals for multi-view microscopy

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Sample chamber originally designed for SiMView

The temperature- and atmosphere-regulated sample chamber designed for the SiMView microscope developed in the Keller Lab is a substantial achievement in addition to the overall microscope innovation. Made to hold a specimen for four objectives, it can be replicated and modified for use with the SiMView microscope, for which designs are also available to researchers, or with other multi-view, multi-objective microscopes.

The chamber was designed to help sustain and image normal mouse embryonic development at high resolution over a period of days, as reported in Cell (In toto imaging and reconstruction of post-implantation mouse development at the single-cell level, McDole et al.), complete with information on the improved SiMView Microscope used in that study.

Opportunity

Designs for the chamber and seals are provided at Flintbox for free under an open-source non-commercial hardware license. The designs include: