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Prior work
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Fly-eFISH builds on several major Team Project and lab collaborations at Janelia.
- The FlyEM Project Team released Hemibrain, Male Adult Nerve Cord (MANC), and Optic Lobe (OL) EM volumes for most of the Drosophila CNS. These volumes describe the morphology of individual neurons and the connectivity between them provided by chemical synapses, providing a neural circuit map.
- The Funke lab developed reliable neurotransmitter (NT) predictions based on details of EM anatomy, describing connection types for the most common form of neural communication.
- The FlyLight Project Team developed a collection of transgenic fly lines using the split-GAL4 system that enable precise targeting of many cell types within the CNS. They have released a collection of 3000 best cell-type-specific split-GAL4 lines.
- The Tillberg lab enhanced the expansion microscopy approach, increasing the effective resolution and clarity of light microscope images by physically expanding samples.
- The multiFISH Project Team developed the EASI-FISH (Expansion-Assisted Iterative Fluorescence In Situ Hybridization) technique to map RNA transcripts in thick sections of expanded samples.
- The Project Technical Resources (PTR) team adapted EASI-FISH to flies and NTs.
- The Rubin lab and PTR further applied EASI-FISH to NPs, focusing on the central complex of the fly brain.